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Literature summary extracted from
- Optimization of adsorption conditions of papain on dye affinity membrane using response surface methodology (2009), Biores. Technol., 100, 2336-2340.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.22.2 | industry | the enzyme with high biological activity and the decomposing ability is widely used in the lines of medical application, cell isolation, food, detergents, leather, textile, cosmetic and pharmaceutical industry | Carica papaya |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.2 | additional information | Carica papaya | enzyme catalyses the hydrolysis of peptide bonds of basic amino acids, such as leucine or glycine | ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.2 | Carica papaya | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.2 | purification of papain using the dye affinity membrane chromatography at pH 7.05 with Tris-HCl, papain is purified 34.6fold in a single step determined by fast protein liquid chromatography | Carica papaya |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.2 | commercial preparation | - |
Carica papaya | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.2 | additional information | enzyme catalyses the hydrolysis of peptide bonds of basic amino acids, such as leucine or glycine | Carica papaya | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.22.2 | papain | - |
Carica papaya |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.2 | 7.1 | - |
The effect of initial pH and temperature on adsorption capacity for papain on the chitosan-coated nylon composite membranes in Tris-HCl buffer is tested, an optimum point of maximum adsorption capacity obtained as 26.61 mg/g at 39°C, pH 7.05 by fixing 8 mg/ml initial papain concentration. Adsorption capacity of papain increases from 26.61 to 27.85 mg/g when the papain concentration increases from 8 to 11 mg/ml. | Carica papaya |
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Release 2023.1 (January 2023)
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