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Literature summary extracted from

  • Dong, C.K.; Patel, V.; Yang, J.C.; Dvorin, J.D.; Duraisingh, M.T.; Clardy, J.; Wirth, D.F.
    Type II NADH dehydrogenase of the respiratory chain of Plasmodium falciparum and its inhibitors (2009), Bioorg. Med. Chem. Lett., 19, 972-975.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.2 expression of His-tagged NDH2 in Escherichia coli Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 additional information no inhibition: dibenziodolium chloride, diphenyliodonium chloride, 1-hydroxy-2-dodecyl-4(1H)quinolone, atovaquone, antimycin A, rotenone, flavone, artemisinin Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.2 0.002
-
 ubiquinone-1 pH 8.0, 25°C Plasmodium falciparum
7.1.1.2 0.0046
-
 oxidized dichlorophenolindophenol pH 8.0, 25°C Plasmodium falciparum
7.1.1.2 0.104
-
 ubiquinone-0 pH 8.0, 25°C Plasmodium falciparum
7.1.1.2 0.239
-
 menadione pH 8.0, 25°C Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.2 Fe signal shape and g values of cluster N4 [4Fe(G)C], are particularly sensitive to the changes of the environment around N4 Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2 recombinant His-tagged pfNDH2 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 additional information no activity with coenzyme Q4 and coenzyme QD Plasmodium falciparum ?
-
 ?
7.1.1.2 NADH + H+ + menadione
-
 Plasmodium falciparum NAD+ + menadiol
-
 ?
7.1.1.2 NADH + H+ + oxidized dichlorophenolindophenol
-
 Plasmodium falciparum NAD+ + reduced dichlorophenolindophenol
-
 ?
7.1.1.2 NADH + H+ + ubiquinone-0 prefers ubiquinol-0 as an acceptor substrate, and can also use the artificial electron acceptors, menadione and dichlorophenol–indophenol Plasmodium falciparum NAD+ + ubiquinol-0
-
 ?
7.1.1.2 NADH + H+ + ubiquinone-1
-
 Plasmodium falciparum NAD+ + ubiquinol-1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 NDH2
-
 Plasmodium falciparum
7.1.1.2 PfNdh2
-
 Plasmodium falciparum
7.1.1.2 type II NADH dehydrogenase
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.1.1.2 25
-
 assay at Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.2 0.015
-
 ubiquinone-1 pH 8.0, 25°C Plasmodium falciparum
7.1.1.2 0.032
-
 oxidized dichlorophenolindophenol pH 8.0, 25°C Plasmodium falciparum
7.1.1.2 0.077
-
 menadione pH 8.0, 25°C Plasmodium falciparum
7.1.1.2 0.33
-
 ubiquinone-0 pH 8.0, 25°C Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.2 7 9
-
 Plasmodium falciparum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
7.1.1.2 6 10 pH 6.0: about 50% of maximal activity, pH 10.0: about 50% of maximal activity Plasmodium falciparum