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Literature summary extracted from
- Cloning, molecular characterization and expression of a cDNA encoding a functional NADH-cytochrome b5 reductase from Mucor racemosus PTCC 5305 in E. coli (2009), Biol. Res., 42, 137-146.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.6.2.2 | His-tag | Mucor racemosus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.2 | 0.013 | - |
ferricyanide | - |
Mucor racemosus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 30000 | - |
x * 30000, SDS-PAGE | Mucor racemosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.2.2 | Mucor racemosus | A8WEG5 | - |
- |
| 1.6.2.2 | Mucor racemosus PTCC 5305 | A8WEG5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.2.2 | - |
Mucor racemosus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 300 | - |
- |
Mucor racemosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.2 | 2 ferricyanide + NADH | - |
Mucor racemosus | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| 1.6.2.2 | 2 ferricyanide + NADH | - |
Mucor racemosus PTCC 5305 | 2 ferrocyanide + NAD+ + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.6.2.2 | ? | x * 30000, SDS-PAGE | Mucor racemosus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.6.2.2 | NADH-cytochrome b5 reductase | - |
Mucor racemosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 40 | - |
- |
Mucor racemosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 7.5 | - |
no significant difference in the pH range of 6-8 | Mucor racemosus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.2 | FAD | - |
Mucor racemosus | |
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Release 2023.1 (January 2023)
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