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Literature summary extracted from

  • Porco, A.; Gamero, E.E.; Mylonas, E.; Isturiz, T.
    Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: cloning and expression of gntP and gntK in Escherichia coli (2008), Biol. Res., 41, 349-358.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.44 gluconate 6-phosphogluconate dehydrogenase, although constituvely expressed, shows a 3-fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions Corynebacterium glutamicum
2.7.1.12 gluconate 6-phosphogluconate dehydrogenase, although constituvely expressed, shows a 3fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.44 in Escherichia coli Corynebacterium glutamicum
2.7.1.12 in Escherichia coli Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.12 18000
-
 predicted from 167 amino acids of gluconate kinase Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Corynebacterium glutamicum
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
2.7.1.12 D-gluconate + ATP Corynebacterium glutamicum
-
 6-phospho-D-gluconate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Corynebacterium glutamicum Q6NHC5
-
-
2.7.1.12 Corynebacterium glutamicum Q8NMT0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 cell culture
-
 Corynebacterium glutamicum
-
2.7.1.12 cell culture
-
 Corynebacterium glutamicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 0.025
-
 activity of 6-phosphogluconate dhydrogenase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37°C, supplemented with glucoronate Corynebacterium glutamicum
1.1.1.44 0.094
-
 activity of 6-phosphogluconate dhydrogenase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37°C, supplemented with 0.5% fructose Corynebacterium glutamicum
1.1.1.44 0.152
-
 activity of 6-phosphogluconate dhydrogenase expressed in transformed Escherichia coli mutants, supplemented with 0.5% fructose as carbon source Corynebacterium glutamicum
1.1.1.44 0.291
-
 activity of 6-phosphogluconate dhydrogenase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37°C, supplemented with 0.5% gluconate Corynebacterium glutamicum
2.7.1.12 0.047
-
 activity of glucokinase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37°C, supplemented with 0.5% gluconate Corynebacterium glutamicum
2.7.1.12 0.388
-
 activity of glucokinase expressed in Escherichia strain M1-8, supplemented with fructose as carbon source Corynebacterium glutamicum
2.7.1.12 1.429
-
 transformed Escherichia coli cells (strain DH5alpha carrying the pTAEK plasmid), grown in Lb and collected during the exponential phase, then cultivated in CAA medium with 0.2% fructose as carbon source Corynebacterium glutamicum
2.7.1.12 2.894
-
 transformed Escherichia coli cells (mutant TGN282 carrying the pTAEK plasmid), grown in Lb and collected during the exponential phase, then cultivated in CAA medium with 0.2% fructose as carbon source Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Corynebacterium glutamicum D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
2.7.1.12 D-gluconate + ATP
-
 Corynebacterium glutamicum 6-phospho-D-gluconate + ADP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.44 6-phosphogluconate dehydrogenase
-
 Corynebacterium glutamicum
1.1.1.44 gnd
-
 Corynebacterium glutamicum
2.7.1.12 gluconokinase
-
 Corynebacterium glutamicum
2.7.1.12 gntK
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.44 37
-
 assay at Corynebacterium glutamicum
2.7.1.12 37
-
 assay at Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
1.1.1.44 metabolism pentose phosphate pathway Corynebacterium glutamicum
2.7.1.12 metabolism pentose phosphate pathway Corynebacterium glutamicum