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Literature summary extracted from

  • Gilch, S.; Meyer, O.; Schmidt, I.
    A soluble form of ammonia monooxygenase in Nitrosomonas europaea (2009), Biol. Chem., 390, 863-873.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.39 Acetylene mechanism-based inhibitor, specifically interacts with catalytically active ammonia monooxygenase Nitrosomonas europaea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.39 cytoplasm the enzyme resides in the cytoplasm of the bacteria in addition to its location in the membrane and is distributed approximately equally in both subcellular fractions Nitrosomonas europaea 5737
-
1.14.99.39 membrane the enzyme resides in the cytoplasm of the bacteria in addition to its location in the membrane and is distributed approximately equally in both subcellular fractions Nitrosomonas europaea 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.39 Cu2+ the enzyme contains Cu (9.4 mol per mol enzyme), Fe (3.9 mol per mol enzyme), and Zn (0.5 to 2.6 mol per mol enzyme) Nitrosomonas europaea
1.14.99.39 Iron the enzyme contains Cu (9.4 mol per mol enzyme), Fe (3.9 mol per mol enzyme), and Zn (0.5 to 2.6 mol per mol enzyme) Nitrosomonas europaea
1.14.99.39 Zinc the enzyme contains Cu (9.4 mol per mol enzyme), Fe (3.9 mol per mol enzyme), and Zn (0.5 to 2.6 mol per mol enzyme) Nitrosomonas europaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.39 24000
-
 3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE Nitrosomonas europaea
1.14.99.39 27000
-
 3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE Nitrosomonas europaea
1.14.99.39 42000
-
 3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE Nitrosomonas europaea
1.14.99.39 283000
-
 non-denaturing gel electrophoresis Nitrosomonas europaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.39 ammonia + AH2 + O2 Nitrosomonas europaea
-
 NH2OH + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.39 Nitrosomonas europaea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.39
-
 Nitrosomonas europaea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.39 0.089
-
 intact cells Nitrosomonas europaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.39 ammonia + AH2 + O2
-
 Nitrosomonas europaea NH2OH + A + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.39 More different from the beta-subunit of membrane-bound ammonia monooxygenase, the beta-subunit of soluble ammonia monooxygenase possesses an N-terminal signal sequence Nitrosomonas europaea
1.14.99.39 nonamer 3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE Nitrosomonas europaea

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.39 cytochrome c1 the gamma-subunit of the alpha3beta3gamma3 enzyme is cytochrome c1 Nitrosomonas europaea