EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.95 | ethyl acrylate | strong inhibitor of the reduction of ethyl vinyl ketone | Clostridium kluyveri | |
1.3.8.1 | diethyl dicarbonate | 1 mM, 83% residual activity | Clostridium kluyveri | |
1.3.8.1 | iodoacetamide | 1 mM, 38% residual activity | Clostridium kluyveri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.95 | 0.00083 | - |
acryloyl-CoA | in 0.1 M phosphate buffer pH 6.4, at 22°C | Clostridium kluyveri | |
1.3.1.95 | 0.008 | - |
(E)-2-butenoyl-CoA | in 0.1 M phosphate buffer pH 6.4, at 22°C | Clostridium kluyveri | |
1.3.1.95 | 0.77 | - |
ethyl vinyl ketone | in 0.1 M phosphate buffer pH 6.4, at 22°C | Clostridium kluyveri | |
1.3.1.95 | 5.6 | - |
acrolein | in 0.1 M phosphate buffer pH 6.4, at 22°C | Clostridium kluyveri | |
1.3.1.95 | 35 | - |
N-acetyl-S-acryloylcysteamine | in 0.1 M phosphate buffer pH 6.4, at 22°C | Clostridium kluyveri | |
1.3.8.1 | 0.00083 | - |
acrylyl-CoA | 22°C, pH 6.4 | Clostridium kluyveri | |
1.3.8.1 | 0.008 | - |
(E)-2-butenoyl-CoA | 22°C, pH 6.4 | Clostridium kluyveri | |
1.3.8.1 | 0.035 | - |
N-acetyl-S-acryloyl-cysteamine | 22°C, pH 6.4 | Clostridium kluyveri | |
1.3.8.1 | 0.77 | - |
ethyl vinyl ketone | 22°C, pH 6.4 | Clostridium kluyveri | |
1.3.8.1 | 5.6 | - |
acrolein | 22°C, pH 6.4 | Clostridium kluyveri |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.95 | additional information | does not contain iron | Clostridium kluyveri | |
1.3.8.1 | Iron | less than 0.2 g-atom per mol protein | Clostridium kluyveri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.95 | 14200 | - |
2 * 14200, SDS-PAGE | Clostridium kluyveri |
1.3.1.95 | 28400 | - |
gel filtration | Clostridium kluyveri |
1.3.8.1 | 14200 | - |
2 * 14200, SDS-PAGE | Clostridium kluyveri |
1.3.8.1 | 28400 | - |
gel filtration | Clostridium kluyveri |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.95 | Clostridium kluyveri | - |
- |
- |
1.3.8.1 | Clostridium kluyveri | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.3.1.95 | the enzyme exhibits up to 11% and up to 16% residual activity after 19 days of exposure to air at pH 8.0 and 10°C and 30°C, respectively | Clostridium kluyveri |
1.3.8.1 | enzyme is not oxygen-sensitive | Clostridium kluyveri |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.95 | DEAE-Sepharose CL-6B column chromatography, phenyl-Sepharose CL-4B column chromatography, CM-Sephadex A-25 gel filtration, and Sephacryl S-200 gel filtration | Clostridium kluyveri |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.8.1 | 170 | - |
22°C, pH 6.4 | Clostridium kluyveri |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.1.95 | -18°C, 30% (w/v) ethylene glycol in 15 mM phosphate buffer pH 8.0, 1 year, 20% loss of activity | Clostridium kluyveri |
1.3.8.1 | -18°C, 30% ethylene glycol, 15 mM phosphate pH 8.0, 20% loss of activity within 1 year | Clostridium kluyveri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.95 | (E)-2-butenoate + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | (E)-2-butenoyl-CoA + methyl viologen | second best substrate, 45% activity compared to acryloyl-CoA | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | 1-penten-3-one + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | 2-cyclohexen-1-one + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | 2-methylacrolein + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | 4-phenyl-3-buten-2-one + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | acrolein + methyl viologen | - |
Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | acrylic acid + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | acryloyl-CoA + methyl viologen | best substrate | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | butyryl-CoA + 2,6-dichlorophenolindophenol | about 1% of the reduction rate of (E)-2-butenoyl-CoA and methyl viologen | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | diethyl ketone + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | ethyl acrylate + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | ethyl vinyl ketone + methyl viologen | - |
Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | methyl propenyl ketone + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | methyl vinyl ketone + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | additional information | the enzyme shows no activity with different concentrations of NAD(P)H in 0.1 M phosphate buffer pH 7.0 and ethyl vinyl ketone as substrate | Clostridium kluyveri | ? | - |
? | |
1.3.1.95 | N-acetyl-S-acryloylcysteamine + methyl viologen | - |
Clostridium kluyveri | ? | - |
? | |
1.3.8.1 | (E)-2-butenoyl-CoA + reduced methylviologen | - |
Clostridium kluyveri | ? + oxidized methylviologen | - |
? | |
1.3.8.1 | acrolein + reduced methylviologen | - |
Clostridium kluyveri | ? + oxidized methylviologen | - |
? | |
1.3.8.1 | acryloyl-CoA + reduced methylviologen | - |
Clostridium kluyveri | propanoyl-CoA + oxidized methylviologen | - |
? | |
1.3.8.1 | ethyl vinyl ketone + reduced methylviologen | - |
Clostridium kluyveri | ? + oxidized methylviologen | - |
? | |
1.3.8.1 | N-acetyl-S-acryloyl-cysteamine + reduced methylviologen | - |
Clostridium kluyveri | ? + oxidized methylviologen | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.95 | homodimer | 2 * 14200, SDS-PAGE | Clostridium kluyveri |
1.3.8.1 | dimer | 2 * 14200, SDS-PAGE | Clostridium kluyveri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.95 | acryloyl-CoA reductase | - |
Clostridium kluyveri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.95 | 5.9 | - |
two optima at pH 5.9 and 6.4 | Clostridium kluyveri |
1.3.1.95 | 6.4 | - |
two optima at pH 5.9 and 6.4 | Clostridium kluyveri |
1.3.8.1 | 5.9 | - |
and 6.4 | Clostridium kluyveri |
1.3.8.1 | 6.4 | - |
and 5.9 | Clostridium kluyveri |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.95 | 3.9 | 8.3 | the activity of the acryloyl-CoA reductase decreases to about 30% at pH 3.9 or pH 8.3, respectively | Clostridium kluyveri |
1.3.8.1 | 3.9 | - |
30% resiudal activity | Clostridium kluyveri |
1.3.8.1 | 8.3 | - |
30% residual activity | Clostridium kluyveri |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.3.8.1 | additional information | - |
enzyme is more stable at pH 8.0 than at pH 6.0 | Clostridium kluyveri |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.95 | FMN | contains 1.5 mol FMN | Clostridium kluyveri | |
1.3.1.95 | additional information | he activity of the acryloyl-CoA reductase is not altered significantly by any of the following substances added in concentrations of 0.075 to 0.15 mM: FMN, FAD, AMP, ADP, ATP, NAD+, NADH, acetyl phosphate and co-enzyme A | Clostridium kluyveri | |
1.3.8.1 | FMN | about 1.5 per mol of dimer | Clostridium kluyveri |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.3.1.95 | Clostridium kluyveri | isoelectric focusing | - |
7.5 |
1.3.8.1 | Clostridium kluyveri | polyacrylamide slab gel | - |
6.9 |
1.3.8.1 | Clostridium kluyveri | chromatofocusing | - |
7.5 |