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Literature summary extracted from

  • Yang, Y.; Yuan, S.; Chen, T.; Ma, P.; Shang, G.; Dai, Y.
    Cloning, heterologous expression, and functional characterization of the nicotinate dehydrogenase gene from Pseudomonas putida KT2440 (2009), Biodegradation, 20, 541-549.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.5 expressed in Pseudomonas entomophila, subcloning of mutants in Escherichia coli failed Pseudomonas putida
1.17.2.1 expression of gene cluster ndhSL in Pseudomonas entomophila Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.2.1 Molybdenum subunit NdhL contains a molybdenum cofactor Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.5 130000
-
 SDS-PAGE Pseudomonas putida
1.17.2.1 16685
-
 x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE Pseudomonas putida
1.17.2.1 127874
-
 x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE Pseudomonas putida
1.17.2.1 130000
-
 x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE Pseudomonas putida
1.17.2.1 130000
-
 x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.5 Pseudomonas putida Q88FX8
-
-
1.17.1.5 Pseudomonas putida KT 2240 Q88FX8
-
-
1.17.2.1 Pseudomonas putida Q88FX8 subunit ndhL; KT2440
-
1.17.2.1 Pseudomonas putida Q88FX9 subunit ndhS; KT2440
-
1.17.2.1 Pseudomonas putida KT 2240 Q88FX8 subunit ndhL; KT2440
-
1.17.2.1 Pseudomonas putida KT 2240 Q88FX9 subunit ndhS; KT2440
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.5 additional information
-
 1.18 micromol/min/mg protein (cells free extract) Pseudomonas putida
1.17.1.5 additional information
-
 1.89 micromol/min/mg dry weight for resting cells Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.5 nicotinate + H2O + electron acceptor
-
 Pseudomonas putida 6-hydroxynicotinate + reduced electron acceptor
-
 ?
1.17.1.5 nicotinate + H2O + electron acceptor
-
 Pseudomonas putida KT 2240 6-hydroxynicotinate + reduced electron acceptor
-
 ?
1.17.1.5 nicotinate + H2O + phenazine methosulfate
-
 Pseudomonas putida 6-hydroxynicotinate + reduced phenazine methosulfate
-
 ?
1.17.1.5 nicotinate + H2O + phenazine methosulfate
-
 Pseudomonas putida KT 2240 6-hydroxynicotinate + reduced phenazine methosulfate
-
 ?
1.17.2.1 nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
-
 Pseudomonas putida 6-hydroxynicotinate + ?
-
 ?
1.17.2.1 nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
-
 Pseudomonas putida KT 2240 6-hydroxynicotinate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.2.1 ? x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE Pseudomonas putida
1.17.2.1 ? x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE Pseudomonas putida
1.17.2.1 More subunit NdhL contains a molybdenum cofactor and cytochrome c Pseudomonas putida
1.17.2.1 More subunit NdhS contains a 2Fe-2S iron-sulfur domain Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.17.1.5 nicotinate dehydrogenase
-
 Pseudomonas putida
1.17.2.1 ndhSL
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.2.1 2Fe-2S-center
-
 Pseudomonas putida
1.17.2.1 cytochrome c
-
 Pseudomonas putida