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Literature summary extracted from

  • da Silva, M.; Alvarez, P.
    Indole-based assay to assess the effect of ethanol on Pseudomonas putida F1 dioxygenase activity (2009), Biodegradation, 2009, 1-6.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.12.11 ethanol ethanol affects toluene aerobic biodegradation activity and exhibits catabolite repression of the enzyme, although the presence of ethanol does not fully repress TDO activity in vivo when toluene is also available as a carbon source Pseudomonas putida F1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.11 indole + NADH + H+ + O2 Pseudomonas putida F1 toluene dioxygenase is ubiquitous in nature and has a broad substrate range, including benzene, toluene, ethylbenzene and xylenes. The organism produces indigo from indole through the activity of TDO 2,2'-bis(2,3-dihydro-3-oxoindolyliden) + NAD+ i.e. indigo ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida F1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 indole + NADH + H+ + O2
-
 Pseudomonas putida F1 2,2'-bis(2,3-dihydro-3-oxoindolyliden) + NAD+ i.e. indigo ?
1.14.12.11 indole + NADH + H+ + O2 toluene dioxygenase is ubiquitous in nature and has a broad substrate range, including benzene, toluene, ethylbenzene and xylenes. The organism produces indigo from indole through the activity of TDO Pseudomonas putida F1 2,2'-bis(2,3-dihydro-3-oxoindolyliden) + NAD+ i.e. indigo ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.11 TDO
-
 Pseudomonas putida F1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.12.11 28
-
 in vivo assay at Pseudomonas putida F1

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
 Pseudomonas putida F1