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Literature summary extracted from

  • Cissell, K.A.; Rahimi, Y.; Shrestha, S.; Deo, S.K.
    Reassembly of a bioluminescent protein renilla luciferase directed through DNA hybridization (2009), Bioconjug. Chem., 20, 15-19.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.5 (1,8-bis-maleimidotriethylene glycol) effective conjugation of the N-terminal and C-terminal fragments of Rluc, results in enzymatic activity Renilla sp.
1.13.12.5 additional information hybridization of two oligonucleotide probes with fused Rluc fragments resulted in reassembly of the fragment, generating active Rluc Renilla sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 expression in Escherichia coli strain ER 2566 Renilla sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.5 G229stopstop Rluc N-terminal fragment Renilla sp.
1.13.12.5 RL8 mutant of Rluc Renilla sp.
1.13.12.5 stop230 Rluc C-terminal fragment Renilla sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.5 additional information free oligonucleotide probe that competes to bind to the hybridizied probe-Rluc fragment complex inhibits the reassembly of Rluc, concentration-dependent; no activity of N-terminal or C-terminal Rluc fragments Renilla sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.5 16000
-
 value about, SDS-PAGE, C-terminal fragment Renilla sp.
1.13.12.5 30000
-
 value about, SDS-PAGE, N-terminal fragment Renilla sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Renilla sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.5 Hi-Trap column Renilla sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.5 coelenterazine + O2
-
 Renilla sp. coelenteramide + CO2 + hnu
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.5 monomer
-
 Renilla sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.12.5 Renilla luciferase
-
 Renilla sp.
1.13.12.5 RLuc
-
 Renilla sp.