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Literature summary extracted from

  • Rodrigues, R.S.; da Silva, J.F.; Boldrini Franca, J.; Fonseca, F.P.; Otaviano, A.R.; Henrique Silva, F.; Hamaguchi, A.; Magro, A.J.; Braz, A.S.; dos Santos, J.I.; Homsi-Brandeburgo, M.I.; Fontes, M.R.; Fuly, A.L.; Soares, A.M.; Rodrigues, V.M.
    Structural and functional properties of Bp-LAAO, a new L-amino acid oxidase isolated from Bothrops pauloensis snake venom (2009), Biochimie, 91, 490-501.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.2 DNA and amino acid sequence determination and analysis, phylogenetic analysis Bothrops pauloensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.2 extracellular venom Bothrops pauloensis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 58000
-
 2 * 65000, SDS-PAGE, 2 * 58000, about, sequence calculation Bothrops pauloensis
1.4.3.2 65000
-
 2 * 65000, SDS-PAGE, 2 * 58000, about, sequence calculation Bothrops pauloensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.2 additional information Bothrops pauloensis LAAO shows leishmanicidal, antitumoral and bactericidal activities dose dependently, overview. LAAO induces platelet aggregation in platelet-rich plasma which is inhibited by catalase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Bothrops pauloensis B5AR80
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.2 flavoprotein
-
 Bothrops pauloensis
1.4.3.2 glycoprotein
-
 Bothrops pauloensis

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 native enzyme from venom by cation exchange and hydrophobic interaction chromatography, followed by adosrption chromatography and HPLC Bothrops pauloensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
 Bothrops pauloensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.2 additional information
-
 substrate specificity, overview Bothrops pauloensis
1.4.3.2 60
-
 purified enzyme, substrate L-Ala Bothrops pauloensis
1.4.3.2 110
-
 purified enzyme, substrate L-Val Bothrops pauloensis
1.4.3.2 140
-
 purified enzyme, substrates L-Tyr or L-Glu Bothrops pauloensis
1.4.3.2 310
-
 purified enzyme, substrate L-Trp Bothrops pauloensis
1.4.3.2 335
-
 purified enzyme, substrate L-His Bothrops pauloensis
1.4.3.2 340
-
 purified enzyme, substrate L-Arg Bothrops pauloensis
1.4.3.2 480
-
 purified enzyme, substrate L-Ile Bothrops pauloensis
1.4.3.2 545
-
 purified enzyme, substrate L-Phe Bothrops pauloensis
1.4.3.2 575
-
 purified enzyme, substrate L-Leu Bothrops pauloensis
1.4.3.2 580
-
 purified enzyme, substrate L-Met Bothrops pauloensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-Ala + H2O + O2
-
 Bothrops pauloensis 2-oxopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Arg + H2O + O2
-
 Bothrops pauloensis 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Glu + H2O + O2
-
 Bothrops pauloensis 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.2 L-His + H2O + O2
-
 Bothrops pauloensis 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Ile + H2O + O2
-
 Bothrops pauloensis 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Leu + H2O + O2
-
 Bothrops pauloensis 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2 best substrate Bothrops pauloensis 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Phe + H2O + O2
-
 Bothrops pauloensis phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Trp + H2O + O2
-
 Bothrops pauloensis indol-3-pyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Tyr + H2O + O2
-
 Bothrops pauloensis 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Val + H2O + O2
-
 Bothrops pauloensis 2-oxoisovalerate + NH3 + H2O2
-
 ?
1.4.3.2 additional information LAAO shows leishmanicidal, antitumoral and bactericidal activities dose dependently, overview. LAAO induces platelet aggregation in platelet-rich plasma which is inhibited by catalase Bothrops pauloensis ?
-
 ?
1.4.3.2 additional information substrate specificity, no activity with L-Pro, L-Thr, L-Ser, and L-Cys, overview. Construction of a LAAO structural model of the stereochemistry of substrate binding by the enzyme, overview Bothrops pauloensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 homodimer 2 * 65000, SDS-PAGE, 2 * 58000, about, sequence calculation Bothrops pauloensis
1.4.3.2 More structure analysis and molecular dynamics simulation, overview Bothrops pauloensis

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 LAAO
-
 Bothrops pauloensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.2 25
-
 assay at Bothrops pauloensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 7.2
-
 assay at Bothrops pauloensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD a flavoenzyme Bothrops pauloensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.2 Bothrops pauloensis sequence calculation
-
 6.3