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Literature summary extracted from

  • Du, Y.; Wu, Y.; Cao, X.; Cui, W.; Zhang, H.; Tian, W.; Ji, M.; Holmgren, A.; Zhong, L.
    Inhibition of mammalian thioredoxin reductase by black tea and its constituents: implications for anticancer actions (2009), Biochimie, 91, 434-444.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.9 1-methyl-1-propyl-2-imidazolyl disulfide
-
 Bos taurus
1.8.1.9 1-methyl-1-propyl-2-imidazolyl disulfide
-
 Homo sapiens
1.8.1.9 curcumin
-
 Bos taurus
1.8.1.9 curcumin
-
 Homo sapiens
1.8.1.9 Hg2+
-
 Bos taurus
1.8.1.9 Hg2+
-
 Homo sapiens
1.8.1.9 additional information black tea extract and theaflavins (mixture of theaflavin, theaflavin-3-monogallate, theaflavin-3'-monogallate and theaflavin-3,3'-digallate) inhibit the purified TrxR1 with IC50 44 mg/ml and 21 mg/ml, respectively. Kinetics of theaflavins exhibit a mixed type of competitive and non-competitive inhibition, with Kis 4 mg/ml and Kii 26 mg/ml against coenzyme NADPH, and with Kis 12 mg/ml and Kii 27 mg/ml against substrate 5,5'-dithiobis(2-nitrobenzoic acid) Homo sapiens
1.8.1.9 theaflavin
-
 Bos taurus
1.8.1.9 theaflavin-3'-monogallate
-
 Bos taurus
1.8.1.9 theaflavin-3,3'-digallate
-
 Bos taurus
1.8.1.9 theaflavin-3-monogallate
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 55000
-
 2 * 55000, SDS-PAGE Homo sapiens
1.8.1.9 110000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Bos taurus O62768
-
-
1.8.1.9 Homo sapiens Q16881
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 EAhy 926 cell
-
 Homo sapiens
-
1.8.1.9 HeLa cell
-
 Homo sapiens
-
1.8.1.9 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Bos taurus 2-nitro-5-thiobenzoate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 homodimer 2 * 55000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 thioredoxin reductase
-
 Homo sapiens
1.8.1.9 thioredoxin reductase
-
 Bos taurus
1.8.1.9 TrxR1
-
 Homo sapiens
1.8.1.9 TrxR1
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 NADPH
-
 Homo sapiens
1.8.1.9 NADPH
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.9 additional information
-
 additional information kinetics of theaflavins exhibit a mixed type of competitive and non-competitive inhibition, with Kis 4 mg/ml and Kii 26 mg/ml against coenzyme NADPH, and with Kis 12 mg/ml and Kii 27 mg/ml against substrate 5,5'-dithiobis(2-nitrobenzoic acid) Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.9 additional information
-
 black tea extract and theaflavins (mixture of theaflavin, theaflavin-3-monogallate, theaflavin-3'-monogallate and theaflavin-3,3'-digallate) inhibit the purified TrxR1 with IC50 44 mg/ml and 21 mg/ml, respectively Homo sapiens additional information
1.8.1.9 0.018
-
 in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 Bos taurus theaflavin-3-monogallate
1.8.1.9 0.0191
-
 in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 Bos taurus theaflavin-3'-monogallate
1.8.1.9 0.0216
-
 in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 Bos taurus theaflavin-3,3'-digallate
1.8.1.9 0.06
-
 IC50 above 0.06 mM, in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 Bos taurus theaflavin