Any feedback?
Literature summary extracted from
- Structural and catalytic roles of residues located in beta13 strand and the following beta - turn loop in Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (2009), Biochim. Biophys. Acta, 1790, 231-239.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.73 | additional information | Phe205, Gly207, and Asn208 in the type II turn of the connecting loop may play a role in the catalytic function of beta-glucanase | Fibrobacter succinogenes |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.73 | wild-type and mutants cloned into vector pET26b(+) and expressed in Escherichia coli BL21 (DE3) | Fibrobacter succinogenes |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.73 | D202L | shows a 1.2fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | D202N | exhibits a 1.8fold increase in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | D206M | shows a 1.1fold increase in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | D206N | exhibits a 1.5fold increase in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | D206R | exhibits the highest relative activity at 50°C over 10 min, shows a 1.2fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | F205L | shows a 3.8fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | G201S | shows a 1.5fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | G207N | shows a fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | K200F | is the most heat-sensitive enzyme, retains 72% of activity at 45°C for 10 min, shows a 1.2fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | K200M | shows a 1.1fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | N208G | shows a fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | R209M | shows a 1.1fold increase, in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | T204F | shows a 2.2fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | W203F | exhibits a 2.4fold increase in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | W203R | exhibits a 207fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.73 | Fibrobacter succinogenes | P17989 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.73 | on Ni-NTA affinity column | Fibrobacter succinogenes |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.73 | 70 | - |
mutant W203R | Fibrobacter succinogenes |
| 3.2.1.73 | 969 | - |
mutant G207- | Fibrobacter succinogenes |
| 3.2.1.73 | 1114 | - |
mutant D206R | Fibrobacter succinogenes |
| 3.2.1.73 | 1377 | - |
mutant F205L | Fibrobacter succinogenes |
| 3.2.1.73 | 1406 | - |
mutant D202L | Fibrobacter succinogenes |
| 3.2.1.73 | 1452 | - |
mutant K200M | Fibrobacter succinogenes |
| 3.2.1.73 | 1501 | - |
mutant N208G | Fibrobacter succinogenes |
| 3.2.1.73 | 1652 | - |
mutant R209M | Fibrobacter succinogenes |
| 3.2.1.73 | 1817 | - |
mutant K200F | Fibrobacter succinogenes |
| 3.2.1.73 | 2034 | - |
mutant T204F | Fibrobacter succinogenes |
| 3.2.1.73 | 2065 | - |
wild-type | Fibrobacter succinogenes |
| 3.2.1.73 | 2155 | - |
mutant G201S | Fibrobacter succinogenes |
| 3.2.1.73 | 2962 | - |
mutant D206N | Fibrobacter succinogenes |
| 3.2.1.73 | 3115 | - |
mutant G207N | Fibrobacter succinogenes |
| 3.2.1.73 | 3282 | - |
mutant D206M | Fibrobacter succinogenes |
| 3.2.1.73 | 4081 | - |
mutant D202N | Fibrobacter succinogenes |
| 3.2.1.73 | 8726 | - |
mutant W203F | Fibrobacter succinogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.73 | lichenan + H2O | - |
Fibrobacter succinogenes | ? | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.73 | 1,3-1,4-beta-D-glucanase | - |
Fibrobacter succinogenes |
| 3.2.1.73 | beta-glucanase | - |
Fibrobacter succinogenes |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.73 | 45 | - |
the wild-type maintains more than 85% of the original activity for 10 min | Fibrobacter succinogenes |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.73 | 44 | - |
lichenan | mutant W203R, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 608 | - |
lichenan | mutant G207-, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 699 | - |
lichenan | mutant D206R, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 865 | - |
lichenan | mutant F205L, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 883 | - |
lichenan | mutant D202L, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 911 | - |
lichenan | mutant K200M, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 942 | - |
lichenan | mutant N208G, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 1037 | - |
lichenan | mutant R209M, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 1141 | - |
lichenan | mutant K200F, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 1277 | - |
lichenan | mutant T204F, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 1296 | - |
lichenan | wild-type, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 1353 | - |
lichenan | mutant G201S, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 1860 | - |
lichenan | mutant D206N, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 1955 | - |
lichenan | mutant G207N, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 2060 | - |
lichenan | mutant D206M, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 2562 | - |
lichenan | mutant D202N, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 3.2.1.73 | 5476 | - |
lichenan | mutant W203F, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
