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Literature summary extracted from

  • Ruggiero, A.; Masullo, M.; Ruocco, M.R.; Grimaldi, P.; Lanzotti, M.A.; Arcari, P.; Zagari, A.; Vitagliano, L.
    Structure and stability of a thioredoxin reductase from Sulfolobus solfataricus: a thermostable protein with two functions (2009), Biochim. Biophys. Acta, 1794, 554-562.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.9 wild type and mutant enzymes with or without NADP+, using 12-15% (w/v) polyethylene glycol 2000 monomethyl ether, 0.1 M (NH4)2SO4, and 50 mM sodium acetate (pH 4.6) Saccharolobus solfataricus
1.8.1.9 wild type enzyme in complex with NADP+ and mutant enzyme C147A, using 12-15% (w/v) poly-ethylene glycol 2000 monomethyl ether, 0.1 M (NH4)2SO4 and 50 mM sodium acetate (pH 4.6) Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.9 C147A the mutant exhibits a marginal NADH oxidase activity with FAD canonically bound to the enzyme Saccharolobus solfataricus
1.8.1.9 C147A in the active site of the C147A mutant, which exhibits a marginal NADH oxidase activity, the FAD is canonically bound to the enzyme Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Saccharolobus solfataricus Q97W27
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 disulfide oxidase + NADPH + H+
-
 Saccharolobus solfataricus ?
-
 ?
1.8.1.9 additional information TrxRB3 also displays NADH oxidase activity Saccharolobus solfataricus ?
-
 ?
1.8.1.9 additional information TrxRB3 is endowed with an additional NADH oxidase activity Saccharolobus solfataricus ?
-
 ?
1.8.1.9 thioredoxin + NADP+
-
 Saccharolobus solfataricus thioredoxin disulfide + NADPH + H+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+
-
 Saccharolobus solfataricus thioredoxin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 homodimer x-ray crystallography Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 thioredoxin reductase
-
 Saccharolobus solfataricus
1.8.1.9 TrxRB3
-
 Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Saccharolobus solfataricus
1.8.1.9 NADPH
-
 Saccharolobus solfataricus