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Literature summary extracted from

  • Kim, J.; Moon, C.H.; Jung, S.; Paik, S.R.
    alpha-Synuclein enhances bioluminescent activity of firefly luciferase by facilitating luciferin localization (2009), Biochim. Biophys. Acta, 1794, 309-314.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.7 alpha-synuclein dose-dependent enhancement of oxyluciferin formation, maximum rate of bioluminescence independent upon ATP concentration Photinus pyralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.7 0.074
-
 Luciferin 0.5 mM ATP, in presence of alpha-synuclein Photinus pyralis
1.13.12.7 0.086
-
 ATP 0.094 mM luciferin, in presence of alpha-synuclein Photinus pyralis
1.13.12.7 0.088
-
 Luciferin 0.2 mM ATP, in presence of alpha-synuclein Photinus pyralis
1.13.12.7 0.114
-
 ATP 0.094 mM luciferin, without alpha-synuclein Photinus pyralis
1.13.12.7 0.114
-
 ATP 0.188 mM luciferin, in presence of alpha-synuclein Photinus pyralis
1.13.12.7 0.134
-
 Luciferin 0.15 mM ATP, in presence of alpha-synuclein Photinus pyralis
1.13.12.7 0.157
-
 ATP 0.188 mM luciferin, without alpha-synuclein Photinus pyralis
1.13.12.7 0.188
-
 Luciferin 0.15 mM ATP, without alpha-synuclein Photinus pyralis
1.13.12.7 0.189
-
 Luciferin 0.5 mM ATP, without alpha-synuclein Photinus pyralis
1.13.12.7 0.192
-
 Luciferin 0.2 mM ATP, without alpha-synuclein Photinus pyralis
1.13.12.7 0.197
-
 Luciferin 0.1 mM ATP, without alpha-synuclein Photinus pyralis
1.13.12.7 0.218
-
 Luciferin 0.1 mM ATP, in presence of alpha-synuclein Photinus pyralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.7 Mg2+ required Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 luciferin + ATP + O2
-
 Photinus pyralis oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 firefly luciferase
-
 Photinus pyralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 7.8
-
 assay at Photinus pyralis