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Literature summary extracted from

  • Goni, G.; Zöllner, A.; Lisurek, M.; Velazquez-Campoy, A.; Pinto, S.; Gomez-Moreno, C.; Hannemann, F.; Bernhardt, R.; Medina, M.
    Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368 (2009), Biochim. Biophys. Acta, 1794, 1635-1642.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.8 additional information
-
 additional information KM-value for the deoxicorticosterone 15beta-hydroxylase activity of recombinant BmCYP106A2 when using the NADPH/Anabaena ferredoxin electron donor system and different electron carrier proteins. Use of different AnFld mutants at positions W57, I59 and I92, which alter their aromatic and hydrophobic character, as electron donor to BmCYP106A2 Priestia megaterium
1.14.15.8 0.00314
-
 reduced ferredoxin pH 7.4, 30°C, wild-type ferredoxin from the cyanobacterium Anabaena PCC 7119 Priestia megaterium
1.14.15.8 0.00734
-
 reduced flavodoxin pH 7.4, 30°C, wild-type flavodoxin from the cyanobacterium Anabaena PCC 7119 Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.8 Priestia megaterium Q06069 ATCC 13368, recombinant enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.8 11-deoxycorticosterone + reduced ferredoxin + O2 ferredoxin from the cyanobacterium Anabaena PCC 7119 Priestia megaterium 15beta-hydroxy-11-deoxycorticosterone + oxidized ferredoxin + H2O
-
 ?
1.14.15.8 11-deoxycorticosterone + reduced flavodoxin + O2 flavodoxin from the cyanobacterium Anabaena PCC 7119 Priestia megaterium 15beta-hydroxy-11-deoxycorticosterone + oxidized flavodoxin + H2O
-
 ?
1.14.15.8 deoxycorticosterone + reduced ferredoxin + O2
-
 Priestia megaterium 15beta-hydroxy-11-deoxycorticosterone + oxidized ferredoxin + H2O
-
 r
1.14.15.8 deoxycorticosterone + reduced flavodoxin + O2
-
 Priestia megaterium 15beta-hydroxy-11-deoxycorticosterone + oxidized flavodoxin + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.14.15.8 BmCYP106A2
-
 Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.8 30
-
 assay at Priestia megaterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.8 additional information
-
 additional information turnover-number for the deoxicorticosterone 15beta-hydroxylase activity of recombinant BmCYP106A2 when using the NADPH/Anabaena ferredoxin electron donor system and different electron carrier proteins. Use of different AnFld mutants at positions W57, I59 and I92, which alter their aromatic and hydrophobic character, as electron donor to BmCYP106A2 Priestia megaterium
1.14.15.8 1.34
-
 reduced flavodoxin pH 7.4, 30°C, wild-type flavodoxin from the cyanobacterium Anabaena PCC 7119 Priestia megaterium
1.14.15.8 3.3
-
 reduced ferredoxin pH 7.4, 30°C, wild-type ferredoxin from the cyanobacterium Anabaena PCC 7119 Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.8 7.4
-
 assay at Priestia megaterium