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Literature summary extracted from

  • Zhang, Z.; Bao, R.; Zhang, Y.; Yu, J.; Zhou, C.Z.; Chen, Y.
    Crystal structure of Saccharomyces cerevisiae cytoplasmic thioredoxin reductase Trr1 reveals the structural basis for species-specific recognition of thioredoxin (2009), Biochim. Biophys. Acta, 1794, 124-128.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.9 hanging drop vapor diffusion method, using 0.1 M MES buffer, pH 6.5, 10% (w/v) PEG 20000, at 18°C Saccharomyces cerevisiae
1.8.1.9 hanging drop vapor diffusion method, using 0.1 M MES buffer, pH 6.5, 10% PEG 20000, at 18°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.9 cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.9 thioredoxin disulfide + NADPH + H+ Saccharomyces cerevisiae
-
 thioredoxin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Saccharomyces cerevisiae P29509
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 Ni-NTA affinity column chromatography, gel filtration Saccharomyces cerevisiae
1.8.1.9 Ni-NTA column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Saccharomyces cerevisiae 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+
-
 Saccharomyces cerevisiae thioredoxin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 homodimer x-ray crystallography Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 thioredoxin reductase
-
 Saccharomyces cerevisiae
1.8.1.9 Trr1
-
 Saccharomyces cerevisiae
1.8.1.9 Trr1 isoform Saccharomyces cerevisiae
1.8.1.9 TRR2 isoform Saccharomyces cerevisiae
1.8.1.9 Trx3 isoform Saccharomyces cerevisiae
1.8.1.9 TrxR
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Saccharomyces cerevisiae
1.8.1.9 FAD one FAD molecule is incorporated in each subunit Saccharomyces cerevisiae
1.8.1.9 NADPH
-
 Saccharomyces cerevisiae