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Literature summary extracted from

  • Wu, B.X.; Zeidan, Y.H.; Hannun, Y.A.
    Downregulation of neutral ceramidase by gemcitabine: Implications for cell cycle regulation (2009), Biochim. Biophys. Acta, 1791, 730-739.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 gemcitabine
-
 Mus musculus
3.5.1.23 NCDase siRNA
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 95000
-
 determined by SDS-PAGE and Western blot analysis, the multiple band pattern between about 95 and 135 kDa may be due to the complexity of modifications, such as O-glycosylation and N-glycosylation Mus musculus
3.5.1.23 135000
-
 determined by SDS-PAGE and Western blot analysis, the multiple band pattern between about 95 and 135 kDa may be due to the complexity of modifications, such as O-glycosylation and N-glycosylation Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 additional information Mus musculus ceramidase catalyzes the hydrolysis of ceramide to form sphingosine and free fatty acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.23 glycoprotein
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 endothelial cell line
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 D-erythro-C12-NBD-ceramide + H2O substrate in activity assay Mus musculus D-erythro-sphingosine + 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid
-
 ?
3.5.1.23 additional information ceramidase catalyzes the hydrolysis of ceramide to form sphingosine and free fatty acid Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 ceramidase
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 5
-
 activity assay, for acid ceramidase activity Mus musculus
3.5.1.23 7
-
 activity assay, for neutral ceramidase activity Mus musculus
3.5.1.23 9
-
 activity assay, for alkaline ceramidase activity Mus musculus