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Literature summary extracted from

  • Rovina, P.; Schanzer, A.; Graf, C.; Mechtcheriakova, D.; Jaritz, M.; Bornancin, F.
    Subcellular localization of ceramide kinase and ceramide kinase-like protein requires interplay of their Pleckstrin Homology domain-containing N-terminal regions together with C-terminal domains (2009), Biochim. Biophys. Acta, 1791, 1023-1030.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.138 expressed in COS-1 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.138 C347A by site-directed mutagenesis, mutation severely impairs localization at the Golgi complex resulting in cytosolic accumulation instead Homo sapiens
2.7.1.138 C351A by site-directed mutagenesis, mutation severely impairs localization at the Golgi complex resulting in cytosolic accumulation instead Homo sapiens
2.7.1.138 C354A by site-directed mutagenesis Homo sapiens
2.7.1.138 DELTA1-123 production by site-directed mutagenesis, localization in cytosol Homo sapiens
2.7.1.138 DELTA124-537 production by site-directed mutagenesis, resulted in almost complete accumulation in the nucleus Homo sapiens
2.7.1.138 DELTA219-496 production by site-directed mutagenesis, localization in cytosol and nucleus Homo sapiens
2.7.1.138 DELTA340-537 production by site-directed mutagenesis, significant accumulation into the nucleus, existence of nuclear export signals in the C-terminal part of enzyme, traditional nuclear export signals 511-IEVRVHCQLVRL-522 in the CC3 domain and a class 2 nuclear export signals 347-CRAGCFVC-354 between the CC1 and the CC2 domains Homo sapiens
2.7.1.138 DELTA454-537 production by site-directed mutagenesis, localization in cytosol and nucleus Homo sapiens
2.7.1.138 DELTA514-537 production by site-directed mutagenesis, nuclear localization of ceramide kinase Homo sapiens
2.7.1.138 DELTA520-537 production by site-directed mutagenesis, localization is mostly cytosolic but is also detected in the nucleus Homo sapiens
2.7.1.138 DELTA525-537 production by site-directed mutagenesis, both localization and cellular activity are lost Homo sapiens
2.7.1.138 DELTA528-537 production by site-directed mutagenesis, no effect of localization and activity when assaying at the cellular level with exogenously added substrate, activity is lost after cell lysis in vitro Homo sapiens
2.7.1.138 DELTA533-537 production by site-directed mutagenesis, no effect of localization and activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.138 cytosol
-
 Homo sapiens 5829
-
2.7.1.138 Golgi complex
-
 Homo sapiens
-
-
2.7.1.138 additional information truncation of the PH domain either from the N-terminus or C-terminus results in disruption of the PH domain fold and loss of wild type ceramide kinase localization Homo sapiens
-
-
2.7.1.138 nucleus
-
 Homo sapiens 5634
-
2.7.1.138 plasma membrane
-
 Homo sapiens 5886
-
2.7.1.138 vesicle microtubule-driven vesicles that traffic between the Golgi complex and the plasma membrane Homo sapiens 31982
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.138 ATP + ceramide Homo sapiens
-
 ADP + ceramide 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Homo sapiens Q8TCT0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ATP + ceramide
-
 Homo sapiens ADP + ceramide 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.138 ceramide kinase
-
 Homo sapiens
2.7.1.138 CERK
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.138 7.2
-
 assay at Homo sapiens