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Literature summary extracted from

  • Lepretre, C.; Fleurier, Y.; Martin, E.; Torriglia, A.
    Nuclear export of LEI/L-DNase II by Crm1 is essential for cell survival (2008), Biochim. Biophys. Acta, 1783, 1068-1075.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.22.1 additional information a nuclear export signal on LEI involved in the control of LEI/L-DNase II nuclearization in healthy cells Sus scrofa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.22.1 LEI cDNA subcloned into pZeoSV2+, into pET23d+ in frame with the His-tag, and into pDsRed2-C1 plasmid in frame with the red fluorescent protein fused to its N-term. Expression in Escherichia coli strain BL21(DE3) pLysS. BHK cells transfected with a pZeo vector containing the wild-type or a punctually mutated LEI cDNA Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.22.1 L286A displays a decrease of survival compared to the wild-type Sus scrofa
3.1.22.1 L290A displays no decrease of survival compared to the wild-type Sus scrofa
3.1.22.1 L293A displays a decrease of survival compared to the wild-type Sus scrofa
3.1.22.1 V295A exhibits a significant cell death compared to the wild-type, highest rate of cells with nuclear retention of LEI Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.22.1 cytoplasm LEI is concentrated around the nucleus Homo sapiens 5737
-
3.1.22.1 cytoplasm LEI is concentrated around the nucleus. LEI/L-DNase II nuclear export is leptomycin B sensitive. The export is mediated by Crm1 receptor Cricetulus griseus 5737
-
3.1.22.1 additional information putative leucine-rich nuclear export signal on LEI/L-DNase II sequence, a mandatory condition for binding exportin molecules. The exportin Crm1 interacts with LEI/L-DNase II Sus scrofa
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.22.1 Cricetulus griseus
-
-
-
3.1.22.1 Homo sapiens
-
-
-
3.1.22.1 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.22.1 ARPE-19 cell
-
 Homo sapiens
-
3.1.22.1 HeLa cell
-
 Homo sapiens
-

Synonyms

EC Number Synonyms Comment Organism
3.1.22.1 LEI-derived DNase II
-
 Cricetulus griseus
3.1.22.1 LEI-derived DNase II
-
 Homo sapiens
3.1.22.1 LEI-derived DNase II
-
 Sus scrofa
3.1.22.1 LEI/L-DNase II
-
 Cricetulus griseus
3.1.22.1 LEI/L-DNase II
-
 Homo sapiens
3.1.22.1 LEI/L-DNase II
-
 Sus scrofa