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Literature summary extracted from
- Oxidant-induced formation of a neutral flavosemiquinone in the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio cholerae (2008), Biochim. Biophys. Acta, 1777, 696-702.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.2.1.1 | - |
Vibrio cholerae serotype O1 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.1.1 | 2-n-heptyl-4-hydroxyquinoline-N-oxide | - |
Vibrio cholerae serotype O1 |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.2.1.1 | 21470 | - |
1 * 48624 (NqrA) + 1 * 45357 (NqrB) + 1 * 27619 (NqrC) + 1 * 22837 (NqrD) + 1 * 21470 (NqrE) + 1 * 45067 (Nqrf), one copy of each Nqr subunit is present in the Na+-NQR complex and a theoretical molecular of 212000 Da is calculated for the complex. Taking into account the additional 50-76 kDa of a n-dodecyl beta-D-maltoside micelle which shields the hydrophobic part of the complex from the polar solvent, it is conluded that NQR-1 complex mainly consists of monomeric Na+-NQR, calculated from sequence | Vibrio cholerae serotype O1 |
| 7.2.1.1 | 22837 | - |
1 * 48624 (NqrA) + 1 * 45357 (NqrB) + 1 * 27619 (NqrC) + 1 * 22837 (NqrD) + 1 * 21470 (NqrE) + 1 * 45067 (Nqrf), one copy of each Nqr subunit is present in the Na+-NQR complex and a theoretical molecular of 212000 Da is calculated for the complex. Taking into account the additional 50-76 kDa of a n-dodecyl beta-D-maltoside micelle which shields the hydrophobic part of the complex from the polar solvent, it is conluded that NQR-1 complex mainly consists of monomeric Na+-NQR, calculated from sequence | Vibrio cholerae serotype O1 |
| 7.2.1.1 | 27619 | - |
1 * 48624 (NqrA) + 1 * 45357 (NqrB) + 1 * 27619 (NqrC) + 1 * 22837 (NqrD) + 1 * 21470 (NqrE) + 1 * 45067 (Nqrf), one copy of each Nqr subunit is present in the Na+-NQR complex and a theoretical molecular of 212000 Da is calculated for the complex. Taking into account the additional 50-76 kDa of a n-dodecyl beta-D-maltoside micelle which shields the hydrophobic part of the complex from the polar solvent, it is conluded that NQR-1 complex mainly consists of monomeric Na+-NQR, calculated from sequence | Vibrio cholerae serotype O1 |
| 7.2.1.1 | 45067 | - |
1 * 48624 (NqrA) + 1 * 45357 (NqrB) + 1 * 27619 (NqrC) + 1 * 22837 (NqrD) + 1 * 21470 (NqrE) + 1 * 45067 (Nqrf), one copy of each Nqr subunit is present in the Na+-NQR complex and a theoretical molecular of 212000 Da is calculated for the complex. Taking into account the additional 50-76 kDa of a n-dodecyl beta-D-maltoside micelle which shields the hydrophobic part of the complex from the polar solvent, it is conluded that NQR-1 complex mainly consists of monomeric Na+-NQR, calculated from sequence | Vibrio cholerae serotype O1 |
| 7.2.1.1 | 45357 | - |
1 * 48624 (NqrA) + 1 * 45357 (NqrB) + 1 * 27619 (NqrC) + 1 * 22837 (NqrD) + 1 * 21470 (NqrE) + 1 * 45067 (Nqrf), one copy of each Nqr subunit is present in the Na+-NQR complex and a theoretical molecular of 212000 Da is calculated for the complex. Taking into account the additional 50-76 kDa of a n-dodecyl beta-D-maltoside micelle which shields the hydrophobic part of the complex from the polar solvent, it is conluded that NQR-1 complex mainly consists of monomeric Na+-NQR, calculated from sequence | Vibrio cholerae serotype O1 |
| 7.2.1.1 | 48624 | - |
1 * 48624 (NqrA) + 1 * 45357 (NqrB) + 1 * 27619 (NqrC) + 1 * 22837 (NqrD) + 1 * 21470 (NqrE) + 1 * 45067 (Nqrf), one copy of each Nqr subunit is present in the Na+-NQR complex and a theoretical molecular of 212000 Da is calculated for the complex. Taking into account the additional 50-76 kDa of a n-dodecyl beta-D-maltoside micelle which shields the hydrophobic part of the complex from the polar solvent, it is conluded that NQR-1 complex mainly consists of monomeric Na+-NQR, calculated from sequence | Vibrio cholerae serotype O1 |
| 7.2.1.1 | 110000 | - |
NQR-2 is the smaller subcomplex of the Na+-NQR consists of NqrA-His, NqrF, NqrB and NqrCNQR-2 but lacks the highly hydrophobic NqrD and NqrE subunit, gel filtration | Vibrio cholerae serotype O1 |
| 7.2.1.1 | 306000 | - |
one copy of each Nqr subunit is present in the Na+-NQR complex and a theoretical molecular of 212000 Da is calculated for the complex. Taking into account the additional 5076 kDa of a n-dodecyl beta-D-maltoside micelle which shields the hydrophobic part of the complex from the polar solvent, it is conluded that NQR-1 complex mainly consists of monomeric Na+-NQR, gel filtration | Vibrio cholerae serotype O1 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.1.1 | NADH + H+ + ubiquinone-1 | Vibrio cholerae serotype O1 | the enzyme catalyzes NADH-driven Na+ transport | NAD+ + ubiquinol-1 | - |
? | |
| 7.2.1.1 | NADH + H+ + ubiquinone-1 | Vibrio cholerae serotype O1 ATCC 39315 | the enzyme catalyzes NADH-driven Na+ transport | NAD+ + ubiquinol-1 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.1.1 | Vibrio cholerae serotype O1 | Q9KPS1 and Q9KPS2 and P0C6E0 and Q9X4Q6 and Q9X4Q7 and Q9X4Q8 | Q9KPS1: subunit NqrA, Q9KPS2: subunit NqrB, P0C6E0: subunit NqrC, Q9X4Q6: subunit NqrD, Q9X4Q7: subunit NqrE, Q9X4Q8: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon. | - |
| 7.2.1.1 | Vibrio cholerae serotype O1 ATCC 39315 | Q9KPS1 and Q9KPS2 and P0C6E0 and Q9X4Q6 and Q9X4Q7 and Q9X4Q8 | Q9KPS1: subunit NqrA, Q9KPS2: subunit NqrB, P0C6E0: subunit NqrC, Q9X4Q6: subunit NqrD, Q9X4Q7: subunit NqrE, Q9X4Q8: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon. | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.2.1.1 | recombinant enzyme | Vibrio cholerae serotype O1 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.1.1 | NADH + H+ + ubiquinone-1 | - |
Vibrio cholerae serotype O1 | NAD+ + ubiquinol-1 | - |
? | |
| 7.2.1.1 | NADH + H+ + ubiquinone-1 | the enzyme catalyzes NADH-driven Na+ transport | Vibrio cholerae serotype O1 | NAD+ + ubiquinol-1 | - |
? | |
| 7.2.1.1 | NADH + H+ + ubiquinone-1 | - |
Vibrio cholerae serotype O1 ATCC 39315 | NAD+ + ubiquinol-1 | - |
? | |
| 7.2.1.1 | NADH + H+ + ubiquinone-1 | the enzyme catalyzes NADH-driven Na+ transport | Vibrio cholerae serotype O1 ATCC 39315 | NAD+ + ubiquinol-1 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.2.1.1 | hexamer | 1 * 48624 (NqrA) + 1 * 45357 (NqrB) + 1 * 27619 (NqrC) + 1 * 22837 (NqrD) + 1 * 21470 (NqrE) + 1 * 45067 (Nqrf), one copy of each Nqr subunit is present in the Na+-NQR complex and a theoretical molecular of 212000 Da is calculated for the complex. Taking into account the additional 50-76 kDa of a n-dodecyl beta-D-maltoside micelle which shields the hydrophobic part of the complex from the polar solvent, it is conluded that NQR-1 complex mainly consists of monomeric Na+-NQR, calculated from sequence | Vibrio cholerae serotype O1 |
| 7.2.1.1 | tetramer | 1 * 48624 (NqrA) + 1 * 45357 (NqrB) + 1 * 27619 (NqrC) + 1 * 45067 (Nqrf), NQR-2 is the smaller subcomplex of the Na+-NQR consists of NqrA-His, NqrF, NqrB and NqrCNQR-2 but lacks the highly hydrophobic NqrD and NqrE subunit, calculated from sequence | Vibrio cholerae serotype O1 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.2.1.1 | Na+-NQR | - |
Vibrio cholerae serotype O1 |
| 7.2.1.1 | Na+-translocating NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
| 7.2.1.1 | NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
| 7.2.1.1 | NQR-1 | complex which elutes at an approximate molecular mass of 306 kDa is termed NQR-1. One copy of each Nqr subunit is present in the Na+-NQR (NqrA-His, NqrF, NqrB, NqrC, NqrD and NqrE). NQR-1 mainly consists of monomeric Na+-NQR | Vibrio cholerae serotype O1 |
| 7.2.1.1 | NQR-2 | the smaller subcomplex of the Na+-NQR consists of NqrA-His, NqrF, NqrB and NqrCNQR-2 but lacks the highly hydrophobic NqrD and NqrE subunits | Vibrio cholerae serotype O1 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.2.1.1 | 7.5 | - |
assay at | Vibrio cholerae serotype O1 |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.1.1 | FAD | the isolated enzyme complex contains near-stoichiometric amounts of non-covalently bound FAD (0.78 mol/mol Na+-NQR) and riboflavin (0.70 mol/mol Na+-NQR). The four flavins in the Na+-NQR are converted to the partially or fully reduced state when the enzyme reacts with NADH. Two non-covalently bound flavins (FAD and riboflavin) preferentially act as two-electron mediators, whereas two covalently bound FMNs undergo one-electron transitions (FMN/FMNU(radical)-) | Vibrio cholerae serotype O1 | |
| 7.2.1.1 | FMN | four flavins in the Na+-NQR are converted to the partially or fully reduced state when the enzyme reacts with NADH. Two non-covalently bound flavins (FAD and riboflavin) preferentially act as two-electron mediators, whereas two covalently bound FMNs undergo one-electron transitions (FMN/FMNU(radical)-) | Vibrio cholerae serotype O1 | |
| 7.2.1.1 | NADH | - |
Vibrio cholerae serotype O1 | |
| 7.2.1.1 | NADH | NQR-2 exhibits approximately 15% of the specific NADH dehydrogenase activity of NQR-1 | Vibrio cholerae serotype O1 | |
| 7.2.1.1 | riboflavin | riboflavin detected in NQR-2 is bound to the NqrB or NqrC subunit | Vibrio cholerae serotype O1 | |
| 7.2.1.1 | riboflavin | the isolated enzyme complex contains near-stoichiometric amounts of non-covalently bound FAD (0.78 mol/mol Na+-NQR) and riboflavin (0.70 mol/mol Na+-NQR). The four flavins in the Na+-NQR are converted to the partially or fully reduced state when the enzyme reacts with NADH. Two non-covalently bound flavins (FAD and riboflavin) preferentially act as two-electron mediators, whereas two covalently bound FMNs undergo one-electron transitions (FMN/FMNU(radical)-) | Vibrio cholerae serotype O1 | |
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