Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, L.; Huang, H.; Nguyen, H.H.; Allen, K.N.; Mariano, P.S.; Dunaway-Mariano, D.
    Divergence of biochemical function in the HAD superfamily: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) (2010), Biochemistry, 49, 1072-1081.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.167
-
 Bordetella bronchiseptica
2.7.7.70
-
 Bordetella bronchiseptica
3.1.3.82
-
 Escherichia coli
3.1.3.82
-
 Bordetella bronchiseptica
3.1.3.82
-
 Mesorhizobium loti
3.1.3.83
-
 Bacteroides thetaiotaomicron

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.82 D-glycero-beta-D-manno-heptose 1-phosphate noncompetitive inhibitor Escherichia coli
3.1.3.82 histidinol
-
 Escherichia coli
3.1.3.82 histidinol phosphate noncompetitive inhibitor Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.82 0.005
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.82 0.0069
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bordetella bronchiseptica
3.1.3.82 0.013
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Mesorhizobium loti
3.1.3.82 0.067
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.82 0.28
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bordetella bronchiseptica
3.1.3.82 0.58
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Mesorhizobium loti
3.1.3.82 0.61
-
 D-sedoheptulose 1,7-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.82 1.5
-
 D-fructose 1,6-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.83 0.033
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bacteroides thetaiotaomicron
3.1.3.83 0.086
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.70 D-glycero-beta-D-manno-heptose 1-phosphate + ATP Bordetella bronchiseptica
-
 ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
 ?
3.1.3.83 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O Bacteroides thetaiotaomicron the likely function of the D-glycero-D-mannoheptose 1alpha-GDP pathway of Bacteroides thetaiotaomicron is to generate activated D-glycero-D-manno-heptose units for incorporation into the extracellular capsular polysaccharide D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.167 Bordetella bronchiseptica Q7WGU8 bifunctional protein hldE includes: D-beta-D-heptose 7-phosphate kinase and D-beta-D-heptose 1-phosphate adenosyltransferase
-
2.7.7.70 Bordetella bronchiseptica Q7WF17 bifunctional protein hldE includes: D-beta-D-heptose 7-phosphate kinase and D-beta-D-heptose 1-phosphate adenosyltransferase
-
3.1.3.82 Bordetella bronchiseptica Q7WG29
-
-
3.1.3.82 Escherichia coli P63228
-
-
3.1.3.82 Mesorhizobium loti Q98I56
-
-
3.1.3.83 Bacteroides thetaiotaomicron Q8AAI7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.167
-
 Bordetella bronchiseptica
2.7.7.70
-
 Bordetella bronchiseptica
3.1.3.82
-
 Escherichia coli
3.1.3.82
-
 Bordetella bronchiseptica
3.1.3.82
-
 Mesorhizobium loti
3.1.3.83
-
 Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.167 D-glycero-D-manno-heptose 7-phosphate + ATP the enzyme selectively produces D-glycero-beta-D-mannoheptose 1,7-bisphosphate Bordetella bronchiseptica D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP
-
 ?
2.7.7.70 D-glycero-beta-D-manno-heptose 1-phosphate + ATP
-
 Bordetella bronchiseptica ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
 ?
2.7.7.70 D-glycero-beta-D-manno-heptose 1-phosphate + ATP no product is obtained when GTP, UTP or CTP is substituted for ATP Bordetella bronchiseptica ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
 ?
3.1.3.82 D-fructose 1,6-bisphosphate + H2O poor substrate Escherichia coli D-fructose 1-phosphate + phosphate
-
 ?
3.1.3.82 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O kcat/Km for the beta-anomer is 100fold higher than for the alpha-anomer Escherichia coli D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
-
 ?
3.1.3.82 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O kcat/Km for the beta-anomer is 150fold higher than for the alpha-anomer Bordetella bronchiseptica D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
-
 ?
3.1.3.82 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O kcat/Km for the beta-anomer is 180fold higher than for the alpha-anomer Mesorhizobium loti D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
-
 ?
3.1.3.82 D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O kcat/Km for the beta-anomer is 100fold higher than for the alpha-anomer Escherichia coli D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
 ?
3.1.3.82 D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O kcat/Km for the beta-anomer is 150fold higher than for the alpha-anomer Bordetella bronchiseptica D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
 ?
3.1.3.82 D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O kcat/Km for the beta-anomer is 180fold higher than for the alpha-anomer Mesorhizobium loti D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
 ?
3.1.3.82 D-sedoheptulose 1,7-bisphosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.3.82 additional information no activity with: fructose 6-phosphate, fructose 1-phosphate, alpha-D-glucose 1,6-bisphosphate, beta-D-glucose 1,6-bisphosphate, alpha-D-mannose 1,6-bisphosphate, and beta-D-mannose 1,6-bisphosphate, histidinol Escherichia coli ?
-
 ?
3.1.3.83 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O the likely function of the D-glycero-D-mannoheptose 1alpha-GDP pathway of Bacteroides thetaiotaomicron is to generate activated D-glycero-D-manno-heptose units for incorporation into the extracellular capsular polysaccharide Bacteroides thetaiotaomicron D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
-
 ?
3.1.3.83 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O kcat/Km for the alpha-anomer is 6fold higher than for the alpha-anomer Bacteroides thetaiotaomicron D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
-
 ?
3.1.3.83 D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O kcat/Km for the alpha-anomer is 6fold higher than for the alpha-anomer Bacteroides thetaiotaomicron D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.70 D-glycero-D-manno-heptose-1beta-phosphate adenylyltransferase
-
 Bordetella bronchiseptica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.82 0.039
-
 D-fructose 1,6-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.82 0.51
-
 D-sedoheptulose 1,7-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.82 4.4
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Mesorhizobium loti
3.1.3.82 4.6
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.82 5.9
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bordetella bronchiseptica
3.1.3.82 18
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Mesorhizobium loti
3.1.3.82 22
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bordetella bronchiseptica
3.1.3.82 35.7
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.83 13
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bacteroides thetaiotaomicron
3.1.3.83 29
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bacteroides thetaiotaomicron

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.82 0.06
-
 D-glycero-beta-D-manno-heptose 1-phosphate pH 7.5, 25°C, Ki(slope) Escherichia coli
3.1.3.82 0.15
-
 D-glycero-beta-D-manno-heptose 1-phosphate pH 7.5, 25°C, Ki(intercept) Escherichia coli
3.1.3.82 1
-
 histidinol pH 7.5, 25°C, Ki(slope) Escherichia coli
3.1.3.82 6
-
 histidinol pH 7.5, 25°C, Ki(intercept) Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.82 0.026
-
 D-fructose 1,6-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.82 0.84
-
 D-sedoheptulose 1,7-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.82 7.6
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Mesorhizobium loti
3.1.3.82 21
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bordetella bronchiseptica
3.1.3.82 69
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.82 1385
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Mesorhizobium loti
3.1.3.82 3188
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bordetella bronchiseptica
3.1.3.82 7140
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Escherichia coli
3.1.3.83 151.1
-
 D-glycero-beta-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bacteroides thetaiotaomicron
3.1.3.83 879
-
 D-glycero-alpha-D-manno-heptose 1,7-bisphosphate pH 7.5, 25°C Bacteroides thetaiotaomicron