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Literature summary extracted from

  • Hugo, M.; Turell, L.; Manta, B.; Botti, H.; Monteiro, G.; Netto, L.E.; Alvarez, B.; Radi, R.; Trujillo, M.
    Thiol and sulfenic acid oxidation of AhpE, the one-cysteine peroxiredoxin from Mycobacterium tuberculosis: kinetics, acidity constants, and conformational dynamics (2009), Biochemistry, 48, 9416-9426.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

General Stability

EC Number General Stability Organism
1.11.1.24 addition of 1 mM N-acetylcysteine to AhpE treated with equimolar H2O2 results in a destabilization of the dimer, leading to lower molecular weight species Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 19320
-
 electrospray ionization mass spectrometry Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 H2O2 + reduced thioredoxin
-
 Mycobacterium tuberculosis H2O + oxidized thioredoxin
-
 ?
1.11.1.24 peroxynitrite + reduced thioredoxin AhpE reduces peroxynitrite 2 orders of magnitude faster than H2O2 Mycobacterium tuberculosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 AhpE a 1-Cys peroxiredoxin Mycobacterium tuberculosis
1.11.1.24 alkyl hydroperoxide reductase
-
 Mycobacterium tuberculosis
1.11.1.24 Rv2238c
-
 Mycobacterium tuberculosis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.24 4.5
-
 AhpE is unstable and precipitates under low pH conditions (below pH 4.5) Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.24 thioredoxin
-
 Mycobacterium tuberculosis