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Literature summary extracted from

  • Zhang, P.; Martin, P.D.; Purcarea, C.; Vaishnav, A.; Brunzelle, J.S.; Fernando, R.; Guy-Evans, H.I.; Evans, D.R.; Edwards, B.F.
    Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis (2009), Biochemistry, 48, 766-778.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.2 expression in Escherichia coli strain BL21 Aquifex aeolicus
3.5.2.3 expression in Escherichia coli strain BL21 Aquifex aeolicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.2 complex of aspartate transcarbamoylase and dihydroorotase Aquifex aeolicus
2.1.3.2 noncovalent hexamer of dihydroorotase and ATCase, to 2.3 A resolution. The structure has citrate, bound to the active sites of both enzymes.Six DHO and six ATC chains form a hollow dodecamer, in which the 12 active sites face an internal reaction chamber that is approximately 60 A in diameter and connected to the cytosol by narrow tunnels. The entrances and the interior of the chamber are both electropositive, which suggests that the architecture of this nanoreactor modifies the kinetics of the bisynthase, not only by steric channeling but also by preferential escape of the product, dihydroorotase Aquifex aeolicus
3.5.2.3 complex of aspartate transcarbamoylase and dihydroorotase Aquifex aeolicus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.2 additional information in noncovalent association with dihydroorotase, possible model for mammalian polypeptide chain CPSase/ATCase/DHOase during pyrimidine biosynthesis Aquifex aeolicus
3.5.2.3 additional information in noncovalent association with aspartate transcarbamoylase, possible model for mammalian polypeptide chain CPSase/ATCase/DHOase during pyrimidine biosynthesis Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Aquifex aeolicus O66726
-
-
2.1.3.2 Aquifex aeolicus O66726 in noncovalent association with dihydroorotase, possible model for the mammalian polypeptide chain CPSase/ATCase/DHOase during pyrimidine biosynthesis
-
3.5.2.3 Aquifex aeolicus O66990 in noncovalent association with aspartate transcarbamoylase, possible model for the mammalian polypeptide chain CPSase/ATCase/DHOase during pyrimidine biosynthesis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.3 N-carbamoyl-L-aspartate
-
 Aquifex aeolicus dihydroorotate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.3.2 hexamer noncovalent association with dihydroorotase Aquifex aeolicus
3.5.2.3 hexamer noncovalent association with aspartate transcarbamoylase Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
2.1.3.2 aspartate transcarbamoylase
-
 Aquifex aeolicus
3.5.2.3 dihydroorotase
-
 Aquifex aeolicus