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Literature summary extracted from

  • Boynton, T.O.; Daugherty, L.E.; Dailey, T.A.; Dailey, HA.
    Identification of Escherichia coli HemG as a novel, menadione-dependent flavodoxin with protoporphyrinogen oxidase activity (2009), Biochemistry, 48, 6705-6711.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.3
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.3 additional information Escherichia coli strain SASX38 lacks functional protoporphyrinogen IX dehydrogenase activity and is reported to be deficient in HemG. Sequence analysis of the SASX38 hemG gene reveals a deletion from nucleotide 152 to 470. This results in a predicted HemG protein that remains in frame but lacks 106 internal amino acids, including the long chain insert loop Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.3 0.00376
-
 menadione pH 8.0, 37°C Escherichia coli
1.3.5.3 0.007
-
 protoporphyrinogen IX pH 8.0, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.3 22000
-
 4 * 22000, SDS-PAGE Escherichia coli
1.3.5.3 22500
-
 4 * 22500, calculated from sequence Escherichia coli
1.3.5.3 108000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.3 protoporphyrinogen IX + 3 menadione Escherichia coli the enzyme enables Escherichia coli to synthesize heme in both aerobic and anaerobic environments protoporphyrin IX + 3 menadiol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.3 six histidine-tagged recombinant protein Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.3 protoporphyrinogen IX + 3 menadione the enzyme enables Escherichia coli to synthesize heme in both aerobic and anaerobic environments Escherichia coli protoporphyrin IX + 3 menadiol
-
 ?
1.3.5.3 protoporphyrinogen IX + 3 menadione HemG is specific for protoporphyrinogen. Neither mesoporphyrinogen nor coproporphyrinogen is oxidized by HemG Escherichia coli protoporphyrin IX + 3 menadiol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.3 tetramer 4 * 22000, SDS-PAGE Escherichia coli
1.3.5.3 tetramer 4 * 22500, calculated from sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.3.5.3 HemG protein
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.5.3 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.3 0.281
-
 menadione pH 8.0, 37°C Escherichia coli
1.3.5.3 0.292
-
 protoporphyrinogen IX pH 8.0, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.3 8
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.3 FMN contains FMN Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.5.3 41.7
-
 protoporphyrinogen IX pH 8.0, 37°C Escherichia coli
1.3.5.3 74.73
-
 menadione pH 8.0, 37°C Escherichia coli