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Literature summary extracted from

  • Moradi, A.; Hosseinkhani, S.; Naderi-Manesh, H.; Sadeghizadeh, M.; Alipour, B.S.
    Effect of charge distribution in a flexible loop on the bioluminescence color of firefly luciferases (2009), Biochemistry, 48, 575-582.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.7 expression in Escherichia coli BL21 Photinus pyralis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.7 ins356E green and red emitting light at pH 7.8, at pH 5.5 red-emitting luciferase, 97% increase in specific activity Photinus pyralis
1.13.12.7 ins356K green and red emitting light at pH 7.8, at pH 5.5 red-emitting luciferase, 18% decrease in specific activity Photinus pyralis
1.13.12.7 ins356Q at pH 5.5 red-emitting luciferase, increase in thermostability, 6% decrease in specific activity Photinus pyralis
1.13.12.7 ins356R green and red emitting light at pH 7.8, at pH 5.5 red-emitting luciferase, 12% decrease in specific activtiy Photinus pyralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.7 0.006
-
 D-luciferin mutant ins356E Photinus pyralis
1.13.12.7 0.009
-
 D-luciferin mutant ins356K Photinus pyralis
1.13.12.7 0.011
-
 D-luciferin wild-type luciferase Photinus pyralis
1.13.12.7 0.015
-
 D-luciferin mutant ins356R Photinus pyralis
1.13.12.7 0.018
-
 D-luciferin mutant ins356Q Photinus pyralis
1.13.12.7 0.1
-
 ATP mutant ins356R Photinus pyralis
1.13.12.7 0.105
-
 ATP mutant ins356E Photinus pyralis
1.13.12.7 0.105
-
 ATP mutant ins356Q Photinus pyralis
1.13.12.7 0.11
-
 ATP mutant ins356K Photinus pyralis
1.13.12.7 0.12
-
 ATP wild-type luciferase Photinus pyralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.7 62000
-
 SDS-PAGE Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis P08659 naturally green-emitting luciferase at pH 7.8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.7 Ni-NTA Sepharose, yield of 95% Photinus pyralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 D-luciferin + ATP + O2
-
 Photinus pyralis luciferyl-adenylate + diphosphate
-
 ?
1.13.12.7 luciferyl-adenylate
-
 Photinus pyralis oxyluciferin + hnu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 firefly luciferase
-
 Photinus pyralis
1.13.12.7 Ppy
-
 Photinus pyralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.7 40
-
 complete inactivation, mutant ins356Q still 20% residual activity Photinus pyralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 5.5
-
 shift of emission spectra to red region Photinus pyralis
1.13.12.7 7.8
-
-
 Photinus pyralis