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Literature summary extracted from

  • Guo, Z.F.; Sun, Y.; Zheng, S.; Guo, Z.
    Preferential hydrolysis of aberrant iIntermediates by the type II thioesterase in Escherichia coli nonribosomal enterobactin synthesis: substrate specificities and mutagenic studies on the active-site residues (dagger) (2009), Biochemistry, 48, 1712-1722.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.20 additional information EntH active site consists of Gln48, Gly55, Glu63, Thr64, Ser67, Met68, and Gly82 Escherichia coli K-12

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.20 overexpressed with a C-terminal hexahistidine tag Escherichia coli K-12

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.20 E63A no catalytic activity toward salicylyl-CoA and 2,3-dihydroxybenzoyl-CoA Escherichia coli K-12
3.1.2.20 E63D no catalytic activity toward salicylyl-CoA and 2,3-dihydroxybenzoyl-CoA Escherichia coli K-12
3.1.2.20 E63Q no catalytic activity toward salicylyl-CoA and 2,3-dihydroxybenzoyl-CoA Escherichia coli K-12
3.1.2.20 H54A 229fold decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12
3.1.2.20 M68A 130fold decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12
3.1.2.20 Q48A no catalytic activity toward salicylyl-CoA and 2,3-dihydroxybenzoyl-CoA Escherichia coli K-12
3.1.2.20 Q48N 290fold decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12
3.1.2.20 S67A 140fold decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12
3.1.2.20 S67C decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12
3.1.2.20 T64S 13fold decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.20 0.00425
-
 palmitoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.0375
-
 2,3-dihydroxybenzoyl-CoA mutant H54A, at room temperature Escherichia coli K-12
3.1.2.20 0.116
-
 2,3-dihydroxybenzoyl-EntB wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.161
-
 2,3-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.162
-
 salicylyl-CoA mutant H54A, at room temperature Escherichia coli K-12
3.1.2.20 0.176
-
 salicylyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.19
-
 4-hydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.196
-
 salicylyl-CoA mutant S67C, at room temperature Escherichia coli K-12
3.1.2.20 0.212
-
 3,4-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.219
-
 2,4-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.256
-
 3,5-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.263
-
 salicylyl-CoA mutant S67A, at room temperature Escherichia coli K-12
3.1.2.20 0.265
-
 3-hydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.272
-
 salicylyl-EntB wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.329
-
 2,3-dihydroxybenzoyl-CoA mutant S67A, at room temperature Escherichia coli K-12
3.1.2.20 0.373
-
 salicylyl-CoA mutant M68A, at room temperature Escherichia coli K-12
3.1.2.20 0.383
-
 2,3-dihydroxybenzoyl-CoA mutant T64S, at room temperature Escherichia coli K-12
3.1.2.20 0.454
-
 2,3-dihydroxybenzoyl-CoA mutant S67C, at room temperature Escherichia coli K-12
3.1.2.20 0.475
-
 benzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.505
-
 2,3-dihydroxybenzoyl-CoA mutant Q48N, at room temperature Escherichia coli K-12
3.1.2.20 0.672
-
 salicylyl-CoA mutant T64S, at room temperature Escherichia coli K-12
3.1.2.20 0.729
-
 salicylyl-CoA mutant Q48N, at room temperature Escherichia coli K-12
3.1.2.20 1.42
-
 2,3-dihydroxybenzoyl-CoA mutant M68A, at room temperature Escherichia coli K-12

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.20 54400
-
 gel filtration Escherichia coli K-12

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.20 Escherichia coli K-12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.20 to homogeneity by metal chelating chromatography and gel filtration Escherichia coli K-12

Storage Stability

EC Number Storage Stability Organism
3.1.2.20 -20°C, PBS buffer, pH 7.4, 10% glycerol Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.20 2,3-dihydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 2,3-dihydroxybenzoate
-
 ?
3.1.2.20 2,3-dihydroxybenzoyl-EntB + H2O
-
 Escherichia coli K-12 EntB + 2,3-dihydroxybenzoate
-
 ?
3.1.2.20 2,4-dihydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 2,4-dihydroxybenzoate
-
 ?
3.1.2.20 3,4-dihydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 3,4-dihydroxybenzoate
-
 ?
3.1.2.20 3,5-dihydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 3,5-dihydroxybenzoate
-
 ?
3.1.2.20 3-hydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 3-hydroxybenzoate
-
 ?
3.1.2.20 4-hydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 4-hydroxybenzoate
-
 ?
3.1.2.20 benzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + benzoate
-
 ?
3.1.2.20 additional information EntH is unable to recognize acetyl-CoA, salicylyl-N-acetylcystamine or 2,3-dihydroxybenzoyl-N-acetylcystamine Escherichia coli K-12 ?
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + palmitate
-
 ?
3.1.2.20 salicylyl-CoA + H2O
-
 Escherichia coli K-12 CoA + salicylate
-
 ?
3.1.2.20 salicylyl-EntB + H2O
-
 Escherichia coli K-12 EntB + salicylate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.20 homotetramer
-
 Escherichia coli K-12

Synonyms

EC Number Synonyms Comment Organism
3.1.2.20 EntH
-
 Escherichia coli K-12
3.1.2.20 TEII
-
 Escherichia coli K-12
3.1.2.20 type II thioesterase
-
 Escherichia coli K-12

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.20 0.00217
-
 2,3-dihydroxybenzoyl-CoA mutant H54A, at room temperature Escherichia coli K-12
3.1.2.20 0.00333
-
 2,3-dihydroxybenzoyl-CoA mutant S67C, at room temperature Escherichia coli K-12
3.1.2.20 0.0112
-
 2,3-dihydroxybenzoyl-CoA mutant S67A, at room temperature Escherichia coli K-12
3.1.2.20 0.0155
-
 2,3-dihydroxybenzoyl-CoA mutant Q48N, at room temperature Escherichia coli K-12
3.1.2.20 0.019
-
 salicylyl-CoA mutant H54A, at room temperature Escherichia coli K-12
3.1.2.20 0.0342
-
 2,4-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.0507
-
 salicylyl-CoA mutant S67A, at room temperature Escherichia coli K-12
3.1.2.20 0.051
-
 salicylyl-CoA mutant S67C, at room temperature Escherichia coli K-12
3.1.2.20 0.0678
-
 salicylyl-CoA mutant Q48N, at room temperature Escherichia coli K-12
3.1.2.20 0.0778
-
 salicylyl-CoA mutant M68A, at room temperature Escherichia coli K-12
3.1.2.20 0.138
-
 2,3-dihydroxybenzoyl-CoA mutant T64S, at room temperature Escherichia coli K-12
3.1.2.20 0.14
-
 2,3-dihydroxybenzoyl-EntB wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.154
-
 palmitoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.207
-
 4-hydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.23
-
 3,4-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.372
-
 2,3-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 0.533
-
 2,3-dihydroxybenzoyl-CoA mutant M68A, at room temperature Escherichia coli K-12
3.1.2.20 1.38
-
 salicylyl-CoA mutant T64S, at room temperature Escherichia coli K-12
3.1.2.20 4.63
-
 salicylyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 4.83
-
 salicylyl-EntB wild-type, at room temperature Escherichia coli K-12
3.1.2.20 7.45
-
 3,5-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 10.3
-
 3-hydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
3.1.2.20 13.9
-
 benzoyl-CoA wild-type, at room temperature Escherichia coli K-12