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Literature summary extracted from

  • Tran, Q.K.; Leonard, J.; Black, D.J.; Persechini, A.
    Phosphorylation within an autoinhibitory domain in endothelial nitric oxide synthase reduces the Ca2+ concentrations required for calmodulin to bind and activate the enzyme (2008), Biochemistry, 47, 7557-7566.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 expressed in Escherichia coli BL21(DE3) cells Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.39 additional information deletion of the autoinhibitory domain doubles the maximum calmodulin-dependent enzyme activity and decreases the EC50(Ca2+) values for calmodulin binding and calmodulin-dependent enzyme activation Bos taurus
1.14.13.39 S617D the phosphomimetic substitution doubles the maximum calmodulin-dependent enzyme activity and decreases the EC50(Ca2+) values for calmodulin binding and enzyme activation compared to the wild type enzyme Bos taurus
1.14.13.39 S617D/S635D the maximum calmodulin-dependent synthase activity of S617/635D eNOS is about 2fold higher than the wild type activity Bos taurus
1.14.13.39 S635D the substitution has little or no effect on enzyme activity Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ required for calmodulin to bind and activate the enzyme, maximum calmodulin-dependent activity is measured at 1.5 mM CaCl2 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Bos taurus P29473
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 Ni-NTA Sepharose column chromatography and 2'-5' ADP Sepharose resin column chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
1.14.13.39 -85°C, 50 mM Tris (pH 7.4) with 10% (v/v) glycerol, 100 mM NaCl, 0.2 mM L-arginine, 0.0002 mM 5,6,7,8-tetrahydro-L-biopterin, 0.02 mM dithiothreitol, and 1 mM 2',3'-AMP (mixed isomers), up to 1 month, no detectable loss of catalytic activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Bos taurus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 oxidized cytochrome c + NADPH + O2
-
 Bos taurus reduced cytochrome c + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.39 endothelial nitric oxide synthase
-
 Bos taurus
1.14.13.39 eNOS
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin
-
 Bos taurus
1.14.13.39 Calmodulin maximum calmodulin-dependent activity is measured at 1.5 mM CaCl2, phosphorylation within an autoinhibitory domain in endothelial nitric oxide synthase reduces the Ca2+ concentrations required for calmodulin to bind and activate the enzyme Bos taurus
1.14.13.39 NADPH
-
 Bos taurus