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Literature summary extracted from

  • Paes, G.; Skov, L.K.; ODonohue, M.J.; Remond, C.; Kastrup, J.S.; Gajhede, M.; Mirza, O.
    The structure of the complex between a branched pentasaccharide and Thermobacillus xylanilyticus GH-51 arabinofuranosidase reveals xylan-binding determinants and induced fit (2008), Biochemistry, 47, 7441-7451.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 overexpression in Escherichia coli Thermobacillus xylanilyticus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.55 hanging drop vapor-diffusion method, inactive mutant in complex with a pentasaccharide, X-ray diffraction structure determination and analysis at 2.0 A resolution, space group P 65 2 2, seleno-methionyl derivative of wild-type arabinofuranosidase, X-ray diffraction structure determination and analysis at 2.2 A resolution, space group P 65 2 2, sequence identities and homologies, phylogentic analyses Thermobacillus xylanilyticus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.55 C180A site-directed mutagenesis, reduced thermostability Thermobacillus xylanilyticus
3.2.1.55 E176Q site-directed mutagenesis, inactive mutant Thermobacillus xylanilyticus
3.2.1.55 M94_I103delinsGG site-directed mutagenesis, 70fold less active than the wild-type enzyme Thermobacillus xylanilyticus
3.2.1.55 W99A site-directed mutagenesis, the arabinosyl moiety is not correctly bound in the active site when Trp99 is replaced by Ala Thermobacillus xylanilyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 1.4
-
 p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf, 60°C Thermobacillus xylanilyticus
3.2.1.55 3.2
-
 p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-C180A mutant Thermobacillus xylanilyticus
3.2.1.55 10
-
 p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-M94_I103delinsGG mutant Thermobacillus xylanilyticus
3.2.1.55 45
-
 p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-W99A mutant Thermobacillus xylanilyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 xylan + H2O Thermobacillus xylanilyticus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Thermobacillus xylanilyticus O69262
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Thermobacillus xylanilyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 10.5
-
 Tx-Abf-W99A mutant, 60°C, p-nitrophenyl-alpha-L-arabinofuranose Thermobacillus xylanilyticus
3.2.1.55 27
-
 Tx-Abf-C180A mutant, 60°C, p-nitrophenyl-alpha-L-arabinofuranose Thermobacillus xylanilyticus
3.2.1.55 169
-
 Tx-Abf, 60°C, p-nitrophenyl-alpha-L-arabinofuranose Thermobacillus xylanilyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Thermobacillus xylanilyticus 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Thermobacillus xylanilyticus 4-nitrophenol + L-arabinose
-
 ?
3.2.1.55 xylan + H2O
-
 Thermobacillus xylanilyticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 hexamer dimer of trimers Thermobacillus xylanilyticus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-L-arabinofuranosidase
-
 Thermobacillus xylanilyticus
3.2.1.55 Tx-Abf
-
 Thermobacillus xylanilyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 24
-
 p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-C180A mutant, 60°C Thermobacillus xylanilyticus
3.2.1.55 35
-
 p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-M94_I103delinsGG mutant, 60°C Thermobacillus xylanilyticus
3.2.1.55 165
-
 p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf-W99A mutant, 60°C Thermobacillus xylanilyticus
3.2.1.55 355
-
 p-nitrophenyl-alpha-L-arabinofuranose Tx-Abf, 60°C Thermobacillus xylanilyticus