EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.15 | determined in the metal-free form at 1.9 A resolution, data collection and structure refinement statistics, molscript ribbon representation of the crystal structure | Deinococcus radiodurans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.15 | 0.023 | - |
dCTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.034 | - |
CTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.046 | - |
IDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.051 | - |
dGDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.062 | - |
dATP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.082 | - |
CDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.083 | - |
dGTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.088 | - |
dITP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.092 | - |
GDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.092 | - |
dCDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.105 | - |
TDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.124 | - |
UTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.137 | - |
UDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.138 | - |
TTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.145 | - |
dADP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.147 | - |
ITP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.217 | - |
ADP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.317 | - |
ATP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.394 | - |
GTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.15 | membrane | - |
Deinococcus radiodurans | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.15 | Co2+ | lower activity in presence of Co2+ than in presence of Mn2+ | Deinococcus radiodurans | |
3.6.1.15 | Mg2+ | highest value for Mg2+ requirement in metal profile determined | Deinococcus radiodurans | |
3.6.1.15 | Mn2+ | higher activity in presence of Mn2+ than of Co2+ | Deinococcus radiodurans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.15 | ADP + H2O | Deinococcus radiodurans | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | AMP + phosphate | - |
? | |
3.6.1.15 | ATP + H2O | Deinococcus radiodurans | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | ADP + phosphate | - |
? | |
3.6.1.15 | CDP + H2O | Deinococcus radiodurans | first nudix hydrolase observed to have a marked preference for CDP and CTP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, nucleophilic substitution at the beta-phosphorus | CMP + phosphate | - |
? | |
3.6.1.15 | CTP + H2O | Deinococcus radiodurans | first nudix hydrolase observed to have a marked preference for CDP and CTP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, nucleophilic substitution at the beta-phosphorus | CDP + phosphate | - |
? | |
3.6.1.15 | dADP + H2O | Deinococcus radiodurans | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | dAMP + phosphate | - |
? | |
3.6.1.15 | dATP + H2O | Deinococcus radiodurans | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | dADP + phosphate | - |
? | |
3.6.1.15 | dCDP + H2O | Deinococcus radiodurans | next most preferred substrates with a relative activity of <50% compared to CDP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy | dCMP + phosphate | - |
? | |
3.6.1.15 | dCTP + H2O | Deinococcus radiodurans | next most preferred substrates with a relative activity of <50% compared to CDP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy | dCDP + phosphate | - |
? | |
3.6.1.15 | dGDP + H2O | Deinococcus radiodurans | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | dGMP + phosphate | - |
? | |
3.6.1.15 | dGTP + H2O | Deinococcus radiodurans | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | dGDP + phosphate | - |
? | |
3.6.1.15 | dITP + H2O | Deinococcus radiodurans | - |
dTDP + phosphate | - |
? | |
3.6.1.15 | GDP + H2O | Deinococcus radiodurans | relatively low substrate activity | GMP + phosphate | - |
? | |
3.6.1.15 | GTP + H2O | Deinococcus radiodurans | relatively low substrate activity | GDP + phosphate | - |
? | |
3.6.1.15 | IDP + H2O | Deinococcus radiodurans | activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, low substrate activity | IMP + phosphate | - |
? | |
3.6.1.15 | ITP + H2O | Deinococcus radiodurans | - |
IDP + phosphate | - |
? | |
3.6.1.15 | TDP + H2O | Deinococcus radiodurans | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | TMP + phosphate | - |
? | |
3.6.1.15 | TTP + H2O | Deinococcus radiodurans | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | TDP + phosphate | - |
? | |
3.6.1.15 | UDP + H2O | Deinococcus radiodurans | relative low substrate activity | UMP + phosphate | - |
? | |
3.6.1.15 | UTP + H2O | Deinococcus radiodurans | relative low substrate activity | UDP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.15 | Deinococcus radiodurans | Q9RXX6 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.15 | additional information | - |
identification of residues involved in metal and substrate binding using NMR chemical shift mapping experiments, results mapped onto the crystal structure, substrate binding possibilities and biological function discussed, further biochemical studies necessary to determine if the specificity for CDP and CTP is part of its housekeeping duties | Deinococcus radiodurans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.15 | ADP + H2O | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | Deinococcus radiodurans | AMP + phosphate | - |
? | |
3.6.1.15 | ATP + H2O | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | Deinococcus radiodurans | ADP + phosphate | - |
? | |
3.6.1.15 | CDP + H2O | first nudix hydrolase observed to have a marked preference for CDP and CTP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, nucleophilic substitution at the beta-phosphorus | Deinococcus radiodurans | CMP + phosphate | - |
? | |
3.6.1.15 | CTP + H2O | first nudix hydrolase observed to have a marked preference for CDP and CTP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, nucleophilic substitution at the beta-phosphorus | Deinococcus radiodurans | CDP + phosphate | - |
? | |
3.6.1.15 | dADP + H2O | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | Deinococcus radiodurans | dAMP + phosphate | - |
? | |
3.6.1.15 | dATP + H2O | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | Deinococcus radiodurans | dADP + phosphate | - |
? | |
3.6.1.15 | dCDP + H2O | next most preferred substrates with a relative activity of <50% compared to CDP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy | Deinococcus radiodurans | dCMP + phosphate | - |
? | |
3.6.1.15 | dCTP + H2O | next most preferred substrates with a relative activity of <50% compared to CDP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy | Deinococcus radiodurans | dCDP + phosphate | - |
? | |
3.6.1.15 | dGDP + H2O | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | Deinococcus radiodurans | dGMP + phosphate | - |
? | |
3.6.1.15 | dGTP + H2O | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | Deinococcus radiodurans | dGDP + phosphate | - |
? | |
3.6.1.15 | dITP + H2O | - |
Deinococcus radiodurans | dTDP + phosphate | - |
? | |
3.6.1.15 | GDP + H2O | relatively low substrate activity | Deinococcus radiodurans | GMP + phosphate | - |
? | |
3.6.1.15 | GTP + H2O | relatively low substrate activity | Deinococcus radiodurans | GDP + phosphate | - |
? | |
3.6.1.15 | IDP + H2O | activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, low substrate activity | Deinococcus radiodurans | IMP + phosphate | - |
? | |
3.6.1.15 | ITP + H2O | - |
Deinococcus radiodurans | IDP + phosphate | - |
? | |
3.6.1.15 | TDP + H2O | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | Deinococcus radiodurans | TMP + phosphate | - |
? | |
3.6.1.15 | TTP + H2O | relative substrate activity toward a variety of nucleoside di- and triphosphate substrates | Deinococcus radiodurans | TDP + phosphate | - |
? | |
3.6.1.15 | UDP + H2O | relative low substrate activity | Deinococcus radiodurans | UMP + phosphate | - |
? | |
3.6.1.15 | UTP + H2O | relative low substrate activity | Deinococcus radiodurans | UDP + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.15 | DR_0079 | - |
Deinococcus radiodurans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.15 | 0.06 | - |
IDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.18 | - |
ITP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.22 | - |
dITP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.29 | - |
ATP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.29 | - |
ADP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.3 | - |
dCTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.31 | - |
dATP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.32 | - |
GTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.39 | - |
GDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.4 | - |
dGTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.51 | - |
dGDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.52 | - |
dADP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.53 | - |
UTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.59 | - |
CTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 0.69 | - |
TTP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 1.03 | - |
TDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 1.12 | - |
dCDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 1.35 | - |
UDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans | |
3.6.1.15 | 1.67 | - |
CDP | substrate concentration between 0.01 and 4.0 mM, 37°C | Deinococcus radiodurans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.15 | 9 | - |
hydrolysis of CDP | Deinococcus radiodurans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.15 | 9 | 9.5 | optimal range for hydrolysis of CDP between | Deinococcus radiodurans |