EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.4.25 | overexpression and purification of wild type and site-directed variants of GCH II | Streptomyces coelicolor |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.4.25 | D127A | about 10fold less efficient than the wild-type protein | Streptomyces coelicolor |
3.5.4.25 | G209D | about 10fold less efficient than the wild-type protein | Streptomyces coelicolor |
3.5.4.25 | M120A | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120C | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120D | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120E | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120F | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120G | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120H | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120I | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120K | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120N | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120Q | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120S | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120T | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120W | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | M120Y | generated by site-directed mutagenesis | Streptomyces coelicolor |
3.5.4.25 | R83H | mutant substantially impaired in overall activity | Streptomyces coelicolor |
3.5.4.25 | Y123F | inactive | Streptomyces coelicolor |
3.5.4.25 | Y123M | about 10fold less efficient than the wild-type protein, produces exclusively 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate | Streptomyces coelicolor |
3.5.4.25 | Y326F | generated by site-directed mutagenesis, catalytically inactive variant | Streptomyces coelicolor |
3.5.4.25 | Y326M | generated by site-directed mutagenesis, catalytically inactive variant | Streptomyces coelicolor |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.25 | 0.008 | - |
GTP | M120Y variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.008 | - |
GTP | variant M120Y, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.0089 | - |
GTP | M120K variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.011 | - |
GTP | M120Q variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.015 | - |
GTP | M120I variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.019 | - |
GTP | M120F variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.019 | - |
GTP | variant M120F, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.024 | - |
GTP | wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.033 | - |
GTP | M120S variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.048 | - |
GTP | variant Y123M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.05 | - |
GTP | M120H variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.056 | - |
GTP | wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.059 | - |
GTP | M120A variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.063 | - |
GTP | wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.067 | - |
GTP | M120C variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.067 | - |
GTP | M120N variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.085 | - |
GTP | variant D127A, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.092 | - |
GTP | variant R123M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.15 | - |
GTP | M120T variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.19 | - |
GTP | M120G variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 0.23 | - |
GTP | variant G209D, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.25 | Zn2+ | content 1 molecule per monomer enzyme, enzyme requires a zinc ion which presumably activates a water molecule for excision of C-8 of GTP | Streptomyces coelicolor | |
3.5.4.25 | Zn2+ | content of 1 molecule per enzyme monomer, enzyme requires a zinc ion which presumably activates a water molecule for excision of C-8 of GTP | Streptomyces coelicolor |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.4.25 | 46100 | - |
monomer, determined by SDS-PAGE | Streptomyces coelicolor |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.25 | GTP + H2O | Streptomyces coelicolor | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ? | - |
? | |
3.5.4.25 | GTP + H2O | Streptomyces coelicolor | enzyme catalyzes the first step in the biosynthesis of riboflavin | 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ? | - |
? | |
3.5.4.25 | additional information | Streptomyces coelicolor | enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II | ? | - |
? | |
3.5.4.25 | additional information | Streptomyces coelicolor | substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.25 | Streptomyces coelicolor | O88011 | homologue SCO 6655 | - |
3.5.4.25 | Streptomyces coelicolor | Q9EWJ8 | homologue SCO1441 | - |
3.5.4.25 | Streptomyces coelicolor | Q9KYI5 | homologue SCO 2687 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.25 | purification of wild type enzyme and its mutants by nickel affinity chromatography, site-directed variants are expressed in the native state and purified to homogeneity by ion exchange and hydrophobic interaction chromatographic steps | Streptomyces coelicolor |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.25 | GTP + H2O | - |
Streptomyces coelicolor | 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ? | - |
? | |
3.5.4.25 | GTP + H2O | enzyme catalyzes the first step in the biosynthesis of riboflavin | Streptomyces coelicolor | 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ? | - |
? | |
3.5.4.25 | additional information | enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II | Streptomyces coelicolor | ? | - |
? | |
3.5.4.25 | additional information | substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency | Streptomyces coelicolor | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.4.25 | 3,4-dihydroxy-2-butanone 4-phosphate synthase | - |
Streptomyces coelicolor |
3.5.4.25 | GCH II | - |
Streptomyces coelicolor |
3.5.4.25 | GTP cyclohydrolase II | - |
Streptomyces coelicolor |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.25 | 0.42 | - |
GTP | M120D variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 1.2 | - |
GTP | variant Y326F, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 1.8 | - |
GTP | M120W variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 3.18 | - |
GTP | M120E variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 3.18 | - |
GTP | SCO 2687, variant Y123F, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 4.86 | - |
GTP | variant Y326M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 10.8 | - |
GTP | M120H variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 20.4 | - |
GTP | M120T variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 42 | - |
GTP | M120A variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 46.8 | - |
GTP | variant M120F, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 57 | - |
GTP | M120G variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 66 | - |
GTP | M120C variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 78 | - |
GTP | M120I variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 78 | - |
GTP | SCO 2687, variant D127A, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 84 | - |
GTP | M120S variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 84 | - |
GTP | variant M120Y, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 108 | - |
GTP | M120N variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 120 | - |
GTP | M120Q variant, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 150 | - |
GTP | wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 168 | - |
GTP | SCO 2687, variant G209D, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 204 | - |
GTP | SCO 2687, wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 228 | - |
GTP | SCO 2687, variant R123M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 348 | - |
GTP | SCO 2687, variant Y123M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor | |
3.5.4.25 | 432 | - |
GTP | wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol | Streptomyces coelicolor |