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Literature summary extracted from

  • Panay, A.J.; Fitzpatrick, P.F.
    Kinetic isotope effects on aromatic and benzylic hydroxylation by Chromobacterium violaceum phenylalanine hydroxylase as probes of chemical mechanism and reactivity (2008), Biochemistry, 47, 11118-11124.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.1 dithiothreitol in the absence of dithiothreitol the activity of recently purified PheH is 50% of that achieved when dithiothreitol is present Chromobacterium violaceum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.1 expressed in Escherichia coli BL21(DE3) cells Chromobacterium violaceum

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.1 I234D mutant shows decreased kcat value for 6,7-dimethyltetrahydropterin compared to the wild type enzyme Chromobacterium violaceum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.1 EDTA in the absence of added iron in the assay, the EDTA-treated PheH exhibits 3.5% of the L-phenylalanine hydroxylation activity of the metal-containing enzyme Chromobacterium violaceum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.1 Fe2+ PheH is a non-heme iron monooxygenase Chromobacterium violaceum

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Chromobacterium violaceum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.1 ammonium sulfate precipitation and DEAE-Sephacel gel filtration Chromobacterium violaceum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.1 4-methyl-L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2
-
 Chromobacterium violaceum ?
-
 ?
1.14.16.1 L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2
-
 Chromobacterium violaceum L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + 6,7-dimethyltetrahydropterin + O2
-
 Chromobacterium violaceum 4-(hydroxymethyl)phenylalanine + 3-methyltyrosine + H2O + 6,7-dimethyl-dihydropterin
-
 ?
1.14.16.1 L-phenylalanine + 6-methyltetrahydropterin + O2
-
 Chromobacterium violaceum L-tyrosine + 2-amino-4a-hydroxy-7-methyl-5,6,7,8-tetrahydropteridin-4(4aH)-one
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.1 PheH
-
 Chromobacterium violaceum
1.14.16.1 phenylalanine hydroxylase
-
 Chromobacterium violaceum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.16.1 0.9
-
 6,7-dimethyltetrahydropterin mutant enzyme I234D, at 25°C with 50 mM HEPES (pH 7.2), 5 mM dithiothreitol Chromobacterium violaceum
1.14.16.1 1.4
-
 6,7-dimethyltetrahydropterin wild type enzyme, at 25°C with 50 mM HEPES (pH 7.2), 5 mM dithiothreitol Chromobacterium violaceum
1.14.16.1 1.6
-
 6-methyltetrahydropterin wild type enzyme, at 25°C with 50 mM HEPES (pH 7.2), 5 mM dithiothreitol Chromobacterium violaceum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.1 5,6,7,8-tetrahydro-L-biopterin
-
 Chromobacterium violaceum