Literature summary extracted from

Kuntz, D.A.; Tarling, C.A.; Withers, S.G.; Rose, D.R.
Structural analysis of Golgi alpha-mannosidase II inhibitors identified from a focused glycosidase inhibitor screen (2008), Biochemistry, 47, 10058-10068.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.2.1.24	in complex with inhibitors mannoimidazole, glucoimidazole, N-octyl-6-epi-valienamine, gluco-hydroxyiminolactam, and [[(3S,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]amino] N-(4-chlorophenyl)carbamate	Drosophila melanogaster
3.2.1.114	-	Drosophila melanogaster
3.2.1.114	in complex with inhibitors mannoimidazole, glucoimidazole, N-octyl-6-epi-valienamine, gluco-hydroxyiminolactam, and [[(3S,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]amino] N-(4-chlorophenyl)carbamate	Drosophila melanogaster

Inhibitors

EC Number	Inhibitors	Comment	Organism
3.2.1.24	deoxymannojirimycin	-	Drosophila melanogaster
3.2.1.24	gluco-hydroxyiminolactam	-	Drosophila melanogaster
3.2.1.24	glucoimidazole	-	Drosophila melanogaster
3.2.1.24	kifunensine	-	Drosophila melanogaster
3.2.1.24	mannoimidazole	-	Drosophila melanogaster
3.2.1.24	N-octyl-6-epi-valienamine	-	Drosophila melanogaster
3.2.1.24	noeuromycin	-	Drosophila melanogaster
3.2.1.24	swainsonine	-	Drosophila melanogaster
3.2.1.24	[[(3S,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]amino] N-(4-chlorophenyl)carbamate	-	Drosophila melanogaster
3.2.1.114	(3S,4S,5R,6R,E)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-one O-4-chlorophenylcarbamoyl oxime	-	Drosophila melanogaster
3.2.1.114	deoxymannojirimycin	-	Drosophila melanogaster
3.2.1.114	gluco-hydroxyiminolactam	-	Drosophila melanogaster
3.2.1.114	glucoimidazole	-	Drosophila melanogaster
3.2.1.114	kifunensine	-	Drosophila melanogaster
3.2.1.114	mannoimidazole	-	Drosophila melanogaster
3.2.1.114	N-octyl-6-epi-valienamine	-	Drosophila melanogaster
3.2.1.114	noeuromycin	-	Drosophila melanogaster
3.2.1.114	swainsonine	-	Drosophila melanogaster
3.2.1.114	[[(3S,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]amino] N-(4-chlorophenyl)carbamate	-	Drosophila melanogaster

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.2.1.114	Golgi apparatus	-	Drosophila melanogaster	5794	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.2.1.24	Zn2+	-	Drosophila melanogaster
3.2.1.114	Zn2+	-	Drosophila melanogaster

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.24	Drosophila melanogaster	-	lysosomal isoform	-
3.2.1.114	Drosophila melanogaster	Q24451	isoform GMII	-
3.2.1.114	Drosophila melanogaster	Q24451	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.2.1.114	SCHNEIDER-2 cell	-	Drosophila melanogaster	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3.2.1.24	2,5-dinitrophenyl-alpha-D-mannopyranoside + H2O	-	Drosophila melanogaster	2,5-dinitrophenol + D-mannose	-	?
3.2.1.114	2,5-dinitrophenyl-alpha-D-mannopyranoside + H2O	-	Drosophila melanogaster	2,5-dinitrophenol + D-mannose	-	?
3.2.1.114	2,5-dinitrophenyl-alpha-D-mannopyranoside + H2O	pH 5.6, room temperature	Drosophila melanogaster	2,5-dinitrophenol + alpha-D-mannose	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.114	alpha-mannosidase	-	Drosophila melanogaster
3.2.1.114	dGMII	-	Drosophila melanogaster
3.2.1.114	Drosophila GMII	-	Drosophila melanogaster
3.2.1.114	glycoside hydrolase	-	Drosophila melanogaster
3.2.1.114	Golgi alpha-mannosidase II	-	Drosophila melanogaster

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
3.2.1.24	0.0003	-	swainsonine	pH 5.6	Drosophila melanogaster
3.2.1.24	0.02	-	mannoimidazole	pH 5.6	Drosophila melanogaster
3.2.1.24	0.033	-	N-octyl-6-epi-valienamine	pH 5.6	Drosophila melanogaster
3.2.1.24	0.046	-	gluco-hydroxyiminolactam	pH 5.6	Drosophila melanogaster
3.2.1.24	0.07	-	glucoimidazole	pH 5.6	Drosophila melanogaster
3.2.1.24	0.15	-	[[(3S,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]amino] N-(4-chlorophenyl)carbamate	pH 5.6	Drosophila melanogaster
3.2.1.114	0.00002	-	swainsonine	-	Drosophila melanogaster
3.2.1.114	0.00002	-	swainsonine	pH 5.6	Drosophila melanogaster
3.2.1.114	0.002	-	mannoimidazole	-	Drosophila melanogaster
3.2.1.114	0.002	-	mannoimidazole	pH 5.6	Drosophila melanogaster
3.2.1.114	0.013	-	glucoimidazole	-	Drosophila melanogaster
3.2.1.114	0.013	-	glucoimidazole	pH 5.6	Drosophila melanogaster
3.2.1.114	0.017	-	N-octyl-6-epi-valienamine	-	Drosophila melanogaster
3.2.1.114	0.017	-	N-octyl-6-epi-valienamine	pH 5.6	Drosophila melanogaster
3.2.1.114	0.02	-	noeuromycin	-	Drosophila melanogaster
3.2.1.114	0.02	-	noeuromycin	pH 5.6	Drosophila melanogaster
3.2.1.114	0.07	-	gluco-hydroxyiminolactam	-	Drosophila melanogaster
3.2.1.114	0.07	-	gluco-hydroxyiminolactam	pH 5.6	Drosophila melanogaster
3.2.1.114	0.4	-	1-deoxymannojirimycin	-	Drosophila melanogaster
3.2.1.114	0.4	-	deoxymannojirimycin	pH 5.6	Drosophila melanogaster
3.2.1.114	0.52	-	(3S,4S,5R,6R,E)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-one O-4-chlorophenylcarbamoyl oxime	-	Drosophila melanogaster
3.2.1.114	0.52	-	[[(3S,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]amino] N-(4-chlorophenyl)carbamate	pH 5.6	Drosophila melanogaster
3.2.1.114	5	-	kifunensine	pH 5.6	Drosophila melanogaster