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Literature summary extracted from

  • Lanciano, P.; Magalon, A.; Bertrand, P.; Guigliarelli, B.; Grimaldi, S.
    High-stability semiquinone intermediate in nitrate reductase A (NarGHI) from Escherichia coli is located in a quinol oxidation site close to heme bD (2007), Biochemistry, 46, 5323-5329.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.7.5.1 H187Y mutant lacking the distal heme bD, no EPR signal of the semiquinone is observed Escherichia coli
1.7.5.1 H187Y mutant lacks the distal heme bD, no EPR signal of the semiquinone is observed Escherichia coli
1.7.5.1 H56Y a semiquinone is detected in the mutant lacking the proximal heme bP. Its thermodynamic properties and spectroscopic characteristics, as revealed by Q-band EPR and ENDOR spectroscopies, are identical to those observed in the native enzyme Escherichia coli
1.7.5.1 H56Y mutant lacks the distal heme bD, a EPR signal of the semiquinone is observed Escherichia coli
1.7.5.1 H66Y mutant lacking the distal heme bD, no EPR signal of the semiquinone is observed Escherichia coli
1.7.5.1 H66Y mutant lacks the distal heme bD, no EPR signal of the semiquinone is observed Escherichia coli
1.7.5.1 K86A mutation dramatically reduces the rate of oxidation of both menaquinol and ubiquinol analogues Escherichia coli
1.7.5.1 K86A the mutation close to heme bD leads to the loss of the EPR signal of the semiquinone, although both hemes are present, the substitution dramatically reduces the rate of oxidation of both mena and ubiquinol analogues Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.5.1 membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.5.1 nitrate + quinol Escherichia coli first enzyme involved in respiratory denitrification in prokaryotes nitrite + quinone + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.5.1 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.5.1 nitrate + 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinol i.e. decylubiquinol Escherichia coli nitrite + 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone + H2O
-
 ?
1.7.5.1 nitrate + 2-methyl-1,4-naphthoquinol i.e menadiol Escherichia coli nitrite + 2-methyl-1,4-naphthoquinone + H2O
-
 ?
1.7.5.1 nitrate + 2-methyl-1,4-naphthoquinol i.e. menadiol Escherichia coli nitrite + 2-methyl-1,4-naphthoquinone + H2O
-
 ?
1.7.5.1 nitrate + 5-hydroxy-1,4-naphthoquinol i.e. juglone Escherichia coli nitrite + 5-hydroxy-1,4-naphthoquinone + H2O
-
 ?
1.7.5.1 nitrate + 5-hydroxy-1,4-naphthoquinol i.e. reduced form of juglone Escherichia coli nitrite + 5-hydroxy-1,4-naphthoquinone + H2O
-
 ?
1.7.5.1 nitrate + 5-hydroxy-2-methyl-naphthalene-1,4-diol i.e plumbagin Escherichia coli nitrite + 5-hydroxy-2-methyl-naphthalene-1,4-dione + H2O
-
 ?
1.7.5.1 nitrate + 5-hydroxy-2-methyl-naphthalene-1,4-diol i.e. reduced form of plumbagin Escherichia coli nitrite + 5-hydroxy-2-methyl-naphthalene-1,4-dione + H2O
-
 ?
1.7.5.1 nitrate + quinol first enzyme involved in respiratory denitrification in prokaryotes Escherichia coli nitrite + quinone + H2O
-
 ?
1.7.5.1 nitrate + quinol NarGHI strongly stabilizes a semiquinone radical located within the dihemic anchor subunit NarI. The semiquinone is located within the quinol oxidation site QD Escherichia coli nitrite + quinone
-
 ?
1.7.5.1 nitrate + tetramethyl-p-benzoquinol i.e. duroquinol Escherichia coli nitrite + tetramethyl-p-benzoquinone + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.5.1 NarGHI
-
 Escherichia coli
1.7.5.1 nitrate reductase A
-
 Escherichia coli
1.7.5.1 quinol/nitrate oxidoreductase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.5.1 cytochrome NarI is strongly associated with heme bD, Lys86 is required for its stabilization Escherichia coli
1.7.5.1 cytochrome bD NarI is strongly associated with heme bD, Lys86 is required for its stabilization Escherichia coli
1.7.5.1 additional information the semiquinone is located within the quinol oxidation site QD Escherichia coli