EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.13 | ArnA transformylase domain | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.2.13 | hanging drop vapor diffusion method, crystal structure of the ArnA transformylase domain is solved to 1.7 A resolution | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.13 | 10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinopyranose | Escherichia coli | ArnA is a key enzyme in the 4-amino-4-deoxy-L-arabinose-lipid A modification pathway. It is a bifunctional enzyme catalyzing the oxidative decarboxylation of UDP-glucuronic acid to the UDP-4''-ketopentose (UDP-beta-L-threo-pentapyranosyl-4''-ulose) and the N-10-formyltetrahydrofolate-dependent formylation of UDP-4-amino-4-deoxy-L-arabinose. The transformylase activity of the Escherichia coli ArnA is contained in its 300 N-terminal residues | 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinopyranose | - |
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EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.13 | Escherichia coli | P77398 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.13 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.13 | 10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinopyranose | ArnA is a key enzyme in the 4-amino-4-deoxy-L-arabinose-lipid A modification pathway. It is a bifunctional enzyme catalyzing the oxidative decarboxylation of UDP-glucuronic acid to the UDP-4''-ketopentose (UDP-beta-L-threo-pentapyranosyl-4''-ulose) and the N-10-formyltetrahydrofolate-dependent formylation of UDP-4-amino-4-deoxy-L-arabinose. The transformylase activity of the Escherichia coli ArnA is contained in its 300 N-terminal residues | Escherichia coli | 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinopyranose | - |
? | |
2.1.2.13 | 10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinopyranose | ArnA is a key enzyme in the 4-amino-4-deoxy-L-arabinose-lipid A modification pathway. It is a bifunctional enzyme catalyzing the oxidative decarboxylation of UDP-glucuronic acid to the UDP-4''-ketopentose (UDP-beta-L-threo-pentapyranosyl-4''-ulose) and the N-10-formyltetrahydrofolate-dependent formylation of UDP-4-amino-4-deoxy-L-arabinose. The transformylase activity of the Escherichia coli ArnA is contained in its 300 N-terminal residues. A mechanism for the transformylation reaction is proposed, catalyzed by ArnA involving residues N102, H104, and D140 | Escherichia coli | 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinopyranose | - |
? |