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Literature summary extracted from

  • Howard-Jones, A.R.; Walsh, C.T.
    Enzymatic generation of the chromopyrrolic acid scaffold of rebeccamycin by the tandem action of RebO and RebD (2005), Biochemistry, 44, 15652-15663.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.23 expression of N-His6-tagged RebO in Escherichia coli Lentzea aerocolonigenes
1.21.98.2 expressed in Escherichia coli BL21(DE3) cells Lentzea aerocolonigenes
1.21.98.2 overproduced as a C-terminally His6-tagged protein, expression in Escherichia coli Lentzea aerocolonigenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.98.2 Fe contains non-heme iron that is not part of an iron-sulfur center Lentzea aerocolonigenes
1.21.98.2 Iron the enzyme contains 2.93 iron centers per polypeptide Lentzea aerocolonigenes
1.21.98.2 additional information does not contain Zn2+, Co2+, Ni2+, Mn2+, Co2+, and Mg2+ Lentzea aerocolonigenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.23 56000
-
 x * 56000, SDS-PAGE Lentzea aerocolonigenes
1.21.98.2 56000
-
 2 * 56000, SDS-PAGE Lentzea aerocolonigenes
1.21.98.2 110000
-
 gel filtration Lentzea aerocolonigenes
1.21.98.2 113000
-
 MALDI-TOF mass spectrometry Lentzea aerocolonigenes
1.21.98.2 113000
-
 gel filtration, MALDI-TOF mass spectrometry Lentzea aerocolonigenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.23 7-chloro-L-tryptophan + O2 Lentzea aerocolonigenes rebeccamycin biosynthetic pathway 2-imino-3-(7-chloroindol-3-yl)propanoate + H2O2
-
 ?
1.21.98.2 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 Lentzea aerocolonigenes rebeccamycin biosynthetic pathway 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O
-
 ?
1.21.98.2 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 Lentzea aerocolonigenes ATCC 39243 rebeccamycin biosynthetic pathway 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.23 Lentzea aerocolonigenes Q8KHS0
-
-
1.21.98.2 Lentzea aerocolonigenes Q8KHV6
-
-
1.21.98.2 Lentzea aerocolonigenes ATCC 39243 Q8KHV6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.23
-
 Lentzea aerocolonigenes
1.21.98.2 Ni-NTA column chromatography, and Superdex 200 gel filtration Lentzea aerocolonigenes
1.21.98.2 purified as a C-terminally His6-tagged protein Lentzea aerocolonigenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.23 7-chloro-L-tryptophan + O2
-
 Lentzea aerocolonigenes 2-imino-3-(7-chloroindol-3-yl)propanoate + H2O2
-
 ?
1.4.3.23 7-chloro-L-tryptophan + O2 rebeccamycin biosynthetic pathway Lentzea aerocolonigenes 2-imino-3-(7-chloroindol-3-yl)propanoate + H2O2
-
 ?
1.21.98.2 2-imino-3-(7-chloroindol-3-yl)propanoate + O2
-
 Lentzea aerocolonigenes dichlorochromopyrrolate + NH3 + H2O
-
 ?
1.21.98.2 2-imino-3-(7-chloroindol-3-yl)propanoate + O2
-
 Lentzea aerocolonigenes ATCC 39243 dichlorochromopyrrolate + NH3 + H2O
-
 ?
1.21.98.2 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2
-
 Lentzea aerocolonigenes 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O
-
 ?
1.21.98.2 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 rebeccamycin biosynthetic pathway Lentzea aerocolonigenes 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O
-
 ?
1.21.98.2 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2
-
 Lentzea aerocolonigenes ATCC 39243 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O
-
 ?
1.21.98.2 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 rebeccamycin biosynthetic pathway Lentzea aerocolonigenes ATCC 39243 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O
-
 ?
1.21.98.2 additional information RebD acts as an efficient catalase, effecting the disproportionation of hydrogen peroxide to give oxygen and water Lentzea aerocolonigenes ?
-
 ?
1.21.98.2 additional information the enzyme has both catalase and chromopyrrolic acid synthase activity. The enzyme acts as an efficient catalase, effecting the disproportionation of hydrogen peroxide to give oxygen and (presumably) water. The enzyme on its own is incompetent with L-tryptophan Lentzea aerocolonigenes ?
-
 ?
1.21.98.2 additional information RebD acts as an efficient catalase, effecting the disproportionation of hydrogen peroxide to give oxygen and water Lentzea aerocolonigenes ATCC 39243 ?
-
 ?
1.21.98.2 additional information the enzyme has both catalase and chromopyrrolic acid synthase activity. The enzyme acts as an efficient catalase, effecting the disproportionation of hydrogen peroxide to give oxygen and (presumably) water. The enzyme on its own is incompetent with L-tryptophan Lentzea aerocolonigenes ATCC 39243 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.23 ? x * 56000, SDS-PAGE Lentzea aerocolonigenes
1.21.98.2 dimer
-
 Lentzea aerocolonigenes
1.21.98.2 dimer 2 * 56000, SDS-PAGE Lentzea aerocolonigenes

Synonyms

EC Number Synonyms Comment Organism
1.4.3.23 RebO
-
 Lentzea aerocolonigenes
1.21.98.2 RebD
-
 Lentzea aerocolonigenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.23 FAD noncovalently bound, FAD occupancy at 70% Lentzea aerocolonigenes
1.21.98.2 FAD
-
 Lentzea aerocolonigenes
1.21.98.2 heme contains one molecule of heme b per monomer Lentzea aerocolonigenes
1.21.98.2 heme the enzyme contains 1.8 heme centers per polypeptide Lentzea aerocolonigenes