EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.28 | expression in Escherichia coli | Methanocaldococcus jannaschii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.8.28 | S211A | mutant enzyme has the same specific activity as the wild type enzyme | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.28 | EDTA | - |
Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.8.28 | 0.017 | - |
lactyl-2-diphospho-5'-guanosine | pH 7.0, 37°C | Methanocaldococcus jannaschii | |
2.7.8.28 | 0.032 | - |
7,8-didemethyl-8-hydroxy-5-deazariboflavin | pH 7.0, 37°C | Methanocaldococcus jannaschii | |
2.7.8.28 | 0.515 | - |
lactyl-2-diphospho-5'-adenosine | pH 7.0, 37°C | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.28 | Mg2+ | isolated CofD enzyme is not activated by the addition of 0-4 mM MgCl2. The inactivation by EDTA can be completely reversed by the addition of excess Mg2+ but not by the addition of Zn2+ or Mn2+ | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.8.28 | 34400 | - |
2 * 34400, SDS-PAGE | Methanocaldococcus jannaschii |
2.7.8.28 | 66700 | - |
gel filtration | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.28 | lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin | Methanocaldococcus jannaschii | the reaction is the fourth step in the biosynthesis of coenzyme F420 | guanosine 5'-phosphate + coenzyme F420-0 | coenzyme F420-0 is coenzyme F420 without glutamic acid | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.28 | Methanocaldococcus jannaschii | Q58653 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.8.28 | recombinant enzyme | Methanocaldococcus jannaschii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.8.28 | (2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = GMP + coenzyme F420-0 | coenzyme F420-0 is coenzyme F420 without glutamic acid | Methanocaldococcus jannaschii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.8.28 | 0.36 | - |
- |
Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.28 | lactyl-2-diphospho-5'-adenosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin | catalysis does not appear to proceed via a covalent intermediate | Methanocaldococcus jannaschii | adenosine 5'-phosphate + coenzyme F420-0 | coenzyme F420-0 is coenzyme F420 without glutamic acid | ? | |
2.7.8.28 | lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin | the reaction is the fourth step in the biosynthesis of coenzyme F420 | Methanocaldococcus jannaschii | guanosine 5'-phosphate + coenzyme F420-0 | coenzyme F420-0 is coenzyme F420 without glutamic acid | ? | |
2.7.8.28 | lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin | catalysis does not appear to proceed via a covalent intermediate. Lactyl-2-diphospho-5'-guanosine is the preferred substrate. The substrate binding site of CofD is very specific for 7,8-didemethyl-8-hydroxy-5-deazariboflavin | Methanocaldococcus jannaschii | guanosine 5'-phosphate + coenzyme F420-0 | coenzyme F420-0 is coenzyme F420 without glutamic acid | ? | |
2.7.8.28 | additional information | to a lesser extent CofD also catalyzes a number of additional reactions that include the formation of 7,8-didemethyl-8-hydroxy-5-deazariboflavin 5'-phosphate, when the enzyme is incubated with 7,8-didemethyl-8-hydroxy-5-deazariboflavin and GDP, GTP, diphosphate, or tripolyphosphate, and the hydrolysis of F420-0 (F420 without glutamic acids) to 7,8-didemethyl-8-hydroxy-5-deazariboflavin | Methanocaldococcus jannaschii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.8.28 | dimer | 2 * 34400, SDS-PAGE | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.8.28 | 2-phospho-L-lactate transferase | - |
Methanocaldococcus jannaschii |
2.7.8.28 | CofD | - |
Methanocaldococcus jannaschii |
2.7.8.28 | lactyl-2-diphospho-(5')guanosine:7,8-didemethyl-8-hydroxy-5-deazariboflavin 2-phospho-L-lactate transferase | - |
Methanocaldococcus jannaschii |
2.7.8.28 | lactyl-2-diphospho-(5')guanosine:Fo 2-phospho-L-lactate transferase | - |
Methanocaldococcus jannaschii |
2.7.8.28 | LPPG:7,8-didemethyl-8-hydroxy-5-deazariboflavin 2-phospho-L-lactate transferase | - |
Methanocaldococcus jannaschii |
2.7.8.28 | LPPG:Fo 2-phospho-L-lactate transferase | - |
Methanocaldococcus jannaschii |
2.7.8.28 | MJ1256 | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.8.28 | 37 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.8.28 | 80 | - |
24 h, completely stable | Methanocaldococcus jannaschii |
2.7.8.28 | 110 | - |
30 min, complete inactivation | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.8.28 | 7 | - |
assay at | Methanocaldococcus jannaschii |