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Literature summary extracted from

  • Dong, C.J.; Yang, X.D.; Liu, J.Y.
    Enzymatic properties of a recombinant phospholipid hydroperoxide glutathione peroxidase from Momordica charantia and its complementation function in yeast (2009), Biochemistry (Moscow), 74, 502-508.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.12 into the vector pET-28a+ for expression in Escherichia coli BL21DE3 cells, into the vector YEpGAP112 for a functional complementation test in yeast Momordica charantia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.12 0.01113
-
phosphatidylcholine hydroperoxide apparent KM Momordica charantia
1.11.1.12 0.6383
-
H2O2 apparent KM Momordica charantia
1.11.1.12 2.932
-
tert-butyl-hydroperoxide apparent KM Momordica charantia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.12 selenium
-
Momordica charantia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.12 22500
-
His6-McPHGPx fusion protein, determined by SDS-PAGE Momordica charantia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.12 glutathione + a lipid hydroperoxide Momordica charantia
-
glutathione disulfide + lipid + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.12 Momordica charantia Q8W259
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.12 by nickel affinity chromatography Momordica charantia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.12 glutathione + a lipid hydroperoxide
-
Momordica charantia glutathione disulfide + lipid + H2O
-
?
1.11.1.12 glutathione + H2O2
-
Momordica charantia ?
-
?
1.11.1.12 glutathione + phosphatidylcholine hydroperoxide
-
Momordica charantia ?
-
?
1.11.1.12 glutathione + tert-butyl-hydroperoxide
-
Momordica charantia ?
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.12 monomer
-
Momordica charantia

Synonyms

EC Number Synonyms Comment Organism
1.11.1.12 McPHGPx
-
Momordica charantia
1.11.1.12 phospholipid hydroperoxide glutathione peroxidase
-
Momordica charantia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.12 40
-
activity assay Momordica charantia

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.12 30 50
-
Momordica charantia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.12 9
-
activity assay Momordica charantia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.12 8 10.5
-
Momordica charantia

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.12 glutathione
-
Momordica charantia