EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.3.3 | DNA and amino acid sequence determination and analysis, sequence comparison, expression of the enzyme fused to the maltose-binding protein in Escherichia coli strain TB1 | Zea mays |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 78000 | - |
x * 78000, recombinant maltose-binding protein fusion protein, SDS-PAGE | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.3 | D-alanine + H2O + O2 | Zea mays | - |
pyruvate + NH3 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.3 | Zea mays | A0PFJ3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.3.3 | recombinant enzyme fused to the maltose-binding protein from Escherichia coli strain TB1 by maltose affinity chromatography | Zea mays |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.3.3 | leaf | from plants grown on D-alanine as nitrogen source | Zea mays | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.3 | D-alanine + H2O + O2 | - |
Zea mays | pyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-alanine + H2O + O2 | recombinant maltose-binding protein-fusion DAAO | Zea mays | pyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-aspartate + H2O + O2 | recombinant maltose-binding protein-fusion DAAO | Zea mays | ? + NH3 + H2O2 | - |
? | |
1.4.3.3 | additional information | recombinant maltose-binding protein-fusion DAAO can oxidize D-alanine and D-aspartate, but not D-leucine, D-isoleucine, and D-serine | Zea mays | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.3.3 | ? | x * 78000, recombinant maltose-binding protein fusion protein, SDS-PAGE | Zea mays |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.3 | DAAO | - |
Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.3 | FAD | dependent on | Zea mays |