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Literature summary extracted from

  • Sinitsyna, O.A.; Fedorova, E.A.; Vakar, I.M.; Kondratieva, E.G.; Rozhkova, A.M.; Sokolova, L.M.; Bubnova, T.M.; Okunev, O.N.; Chulkin, A.M.; Vinetsky, Y.P.; Sinitsyn, A.P.
    Isolation and characterization of extracellular alpha-galactosidases from Penicillium canescens (2008), Biochemistry (Moscow), 73, 97-106.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.22
-
stachyose pH 5.0, 40°C Penicillium canescens
3.2.1.22 0.48
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 40°C Penicillium canescens
3.2.1.22 0.49
-
raffinose pH 5.0, 40°C Penicillium canescens
3.2.1.22 0.61
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 40°C Penicillium canescens
3.2.1.22 2 3 raffinose pH 5.0, 40°C Penicillium canescens
3.2.1.22 21
-
stachyose pH 5.0, 40°C Penicillium canescens
3.2.1.22 34
-
galactomannan with galactose/mannose ratio 1:2.4 pH 5.0, 50°C Penicillium canescens
3.2.1.22 44
-
galactomannan with galactose/mannose ratio 1:1.72 pH 5.0, 50°C Penicillium canescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 45856
-
x * 61000, SDS-PAGE, x * 45856, calculated Penicillium canescens
3.2.1.22 61000
-
x * 61000, SDS-PAGE, x * 45856, calculated Penicillium canescens
3.2.1.22 80000
-
x * 80000, SDS-PAGE, x * 81858, calculated Penicillium canescens
3.2.1.22 81858
-
x * 80000, SDS-PAGE, x * 81858, calculated Penicillium canescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Penicillium canescens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 3.5
-
pH 5.0, 50°C, substrate galactomannan with galactose/mannose ratio 1:1.72 Penicillium canescens
3.2.1.22 6.2
-
pH 5.0, 50°C, substrate galactomannan with galactose/mannose ratio 1:2.4 Penicillium canescens
3.2.1.22 98
-
pH 5.0, 40°C, substrate 4-nitrophenyl-alpha-D-galactopyranoside Penicillium canescens
3.2.1.22 256
-
pH 5.0, 50°C, substrate raffinose Penicillium canescens
3.2.1.22 256
-
pH 5.0, 50°C, substrate stachyose Penicillium canescens
3.2.1.22 1255
-
pH 5.0, 40°C, substrate 4-nitrophenyl-alpha-D-galactopyranoside Penicillium canescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
Penicillium canescens 4-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 galactomannan + H2O with galactose/mannose ratios of 2.4 and of 1.72 Penicillium canescens ?
-
?
3.2.1.22 galactomannan with galactose/mannose ratio 1:1.72 + H2O
-
Penicillium canescens ?
-
?
3.2.1.22 galactomannan with galactose/mannose ratio 1:2.4 + H2O
-
Penicillium canescens ?
-
?
3.2.1.22 raffinose + H2O
-
Penicillium canescens sucrose + D-galactose
-
?
3.2.1.22 raffinose + H2O
-
Penicillium canescens sucrose + alpha-D-galactose
-
?
3.2.1.22 stachyose + H2O
-
Penicillium canescens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 ? x * 61000, SDS-PAGE, x * 45856, calculated Penicillium canescens
3.2.1.22 ? x * 80000, SDS-PAGE, x * 81858, calculated Penicillium canescens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 AglA
-
Penicillium canescens
3.2.1.22 AglC
-
Penicillium canescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 55
-
-
Penicillium canescens
3.2.1.22 65
-
-
Penicillium canescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 0.57
-
stachyose pH 5.0, 40°C Penicillium canescens
3.2.1.22 0.89
-
raffinose pH 5.0, 40°C Penicillium canescens
3.2.1.22 7
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 40°C Penicillium canescens
3.2.1.22 7
-
galactomannan with galactose/mannose ratio 1:1.72 pH 5.0, 50°C Penicillium canescens
3.2.1.22 10
-
galactomannan with galactose/mannose ratio 1:2.4 pH 5.0, 50°C Penicillium canescens
3.2.1.22 1100
-
raffinose pH 5.0, 40°C Penicillium canescens
3.2.1.22 1250
-
stachyose pH 5.0, 40°C Penicillium canescens
3.2.1.22 1800
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 40°C Penicillium canescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 4 5
-
Penicillium canescens
3.2.1.22 5
-
-
Penicillium canescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 2.5
-
complete loss of activity Penicillium canescens
3.2.1.22 7
-
complete loss of activity Penicillium canescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.22 Penicillium canescens isoelectric focusing
-
4.8
3.2.1.22 Penicillium canescens calculated
-
4.9
3.2.1.22 Penicillium canescens calculated
-
5
3.2.1.22 Penicillium canescens isoelectric focusing
-
5.1