Literature summary extracted from
Koksharov, M.I.; Ugarova, N.N.
Random mutagenesis of Luciola mingrelica firefly luciferase. Mutant enzymes with bioluminescence spectra showing low pH sensitivity (2008), Biochemistry (Moscow), 73, 862-869.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.13.12.7 |
expression in Escherichia coli BL21 and XL1blue |
Luciola mingrelica |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.13.12.7 |
F16L |
lower pH-dependence of bioluminescence spectra, about 40% decrease in specific activity |
Luciola mingrelica |
1.13.12.7 |
F16L/A40S |
bioluminescence spectra independent from pH in range of pH 6.0-7.8, about 80% decrease in specific activity |
Luciola mingrelica |
1.13.12.7 |
S118C |
increased thermostability, about 30% increase in specific activity |
Luciola mingrelica |
1.13.12.7 |
Y35H |
bioluminescence spectra independent from pH in range of pH 6.0-7.8, about 40% decrease in specific activity |
Luciola mingrelica |
1.13.12.7 |
Y35N |
bioluminescence spectra independent from pH in range of pH 6.0-7.8, about 30% decrease in specific activity |
Luciola mingrelica |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.12.7 |
Luciola mingrelica |
Q26304 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.13.12.7 |
gel filtration |
Luciola mingrelica |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.12.7 |
luciferin + ATP + O2 |
- |
Luciola mingrelica |
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.13.12.7 |
firefly luciferase |
- |
Luciola mingrelica |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.13.12.7 |
additional information |
- |
shift in bioluminescence spectra to red region by increase in temperature to 42°C |
Luciola mingrelica |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
1.13.12.7 |
42 |
- |
50% decrease in activity after 6.9 min for wild-type luciferase, for mutant S118C after 13.4 min |
Luciola mingrelica |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.13.12.7 |
7.8 |
- |
assay at, shift in bioluminescence spectra to red region by increase in temperature to 42°C |
Luciola mingrelica |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
1.13.12.7 |
6 |
7.8 |
bioluminescence spectra independent from pH, for mutants Y35N, Y35H and F16L/A40S |
Luciola mingrelica |