Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ilhan, A.; Gartner, W.; Nabokikh, A.; Daneva, T.; Majdic, O.; Cohen, G.; Boehmig, G.A.; Base, W.; Hoerl, W.H.; Wagner, L.
    Localization and characterization of the novel protein encoded by C20orf3 (2008), Biochem. J., 414, 485-495.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.6 molecular biology postulated PON (paraoxonase) family membership due to similarity to primary structure of PON2, plant strictosidine synthase and di-isopropyl fluorophosphatase, its N-terminal single transmembrane domain, a nine-exon gene structure, a six-bladed beta-propeller tertiary structure, and similar metabolic regulation of gene expression Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.6 cDNA of 1248 bp in pGEM-T-easy for sequencing (polymorphic residues at position 65, 282, 374, in middle European population homozygosity for R282 and R347) and subcloning, cDNA coding for residues 45-416 in pDEST15 or residues 58-416 in pGEX-1gammaT (yielding glutathione transferase-tag) for expression in Escherichia coli BL21, cDNA coding for residues 16-416 in pDEST10 for baculovirus-mediated expression in insect cell lines Sf9 and HighFive with N-terminal hexa-His-tag Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.6 structural modelling using Cn3D 4.1 software and plant strictosidine synthase as model structure, six-bladed beta-propeller structure Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.6 EDTA metal-chelating reduces enzymatic activity by ca. 33% Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.6 cell membrane heavy membrane after sucrose density gradient cell fractionation on Hep-G2 (full-length protein and 32 kDa variant) and RINm5F cells (full-length protein) Homo sapiens
-
-
3.1.1.6 cytosol 32 kDa low molecular weight variant, sucrose density gradient cell fractionation on RINm5F cells Homo sapiens 5829
-
3.1.1.6 extracellular C-terminus of full-length protein (residues 62-416) as revealed by cell surface domain-specific FACS analyses on monocytes and Hep-G2 cells as well as cell surface domain-specific cytochemistry and confocal microscopy on liver, kidney, and pancreas, detection by ELISA in urine and supernatant of thrombin-treated Hep-G2 cells Homo sapiens
-
-
3.1.1.6 additional information not detectable by ELISA using cell surface domain-specific antibody Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.6 30000
-
 degradation product, Western blot, not detectable by cytosolic domain-specific antibody Homo sapiens
3.1.1.6 32000
-
 low molecular variant, membrane associated (Hep-G2 cell) or cytosolic (RINm5F cell), Western blot Homo sapiens
3.1.1.6 50000
-
 full-length protein, Western blot Homo sapiens
3.1.1.6 52000
-
 high molecular variant in Hep-G2 and SH-SY5Y cells, Western blot Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.6 glycoprotein residues N160 and N196 are potential sites, in silico analysis Homo sapiens
3.1.1.6 additional information residues C140, C149 and C409 with putative intra- and intermolecular disulfide bonds, in silico analysis Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.6 from bacterial lysate or inclusion bodies by Superose 6 column chromatography in presence of 6-8 M urea for antibody generation, from insect cells by FPLC-based metal affinity chromatography (250 mM imidazole) and anion-exchange chromatography on Q-sepharose column for activity measurements Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.6 adult ubiquitous transcript of ca. 2.4 kb, highest gene expression level in liver, placenta and heart, Northern blot Homo sapiens
-
3.1.1.6 artery wall kidney, cytochemistry and confocal microscopy Homo sapiens
-
3.1.1.6 embryo ubiquitous transcript of ca. 2.4 kb, highest gene expression level in liver and kidney, Northern blot Homo sapiens
-
3.1.1.6 endothelial cell kidney, cytochemistry and confocal microscopy Homo sapiens
-
3.1.1.6 HEK-293 cell lower expression level than in Hep-G2 cells as revealed by Northern blot and quantitative RT-PCR, serum-induced inhibition of gene expression (quantitative RT-PCR, FACS, reporter gene assay) Homo sapiens
-
3.1.1.6 Hep-G2 cell strong expression, lower expression level than in liver as revealed by Northern blot and quantitative RT-PCR, cell fractionation, FACS analysis Homo sapiens
-
3.1.1.6 kidney lower expression level than in liver as revealed by Northern blot and quantitative RT-PCR, Western blot, strong in glomeruli and tubular structures according to cytochemistry and confocal microscopy Homo sapiens
-
3.1.1.6 leukocyte no expression in granulocytes and lymphocytes according to cell surface domain-specific FACS analyses Homo sapiens
-
3.1.1.6 liver highest expression level as revealed by Northern blot and quantitative RT-PCR, Western blot, cytochemistry and confocal microscopy Homo sapiens
-
3.1.1.6 pancreatic islet highest expression within pancreatic tissue at the islets of Langerhans, revealed by cytochemistry and confocal microscopy Homo sapiens
-
3.1.1.6 peripheral blood mononuclear cell high expression on monocytes according to cell-surface domain-specific FACS analyses Homo sapiens
-
3.1.1.6 placenta high expression level, Northern blot, quantitative RT-PCR Homo sapiens
-
3.1.1.6 SH-SY5Y cell quantitative RT-PCR, Western blot Homo sapiens
-
3.1.1.6 urine ELISA using cell surface domain-specific antibody Homo sapiens
-
3.1.1.6 vascular smooth muscle cell kidney, cytochemistry and confocal microscopy Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 beta-naphthyl acetate + H2O sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8 Homo sapiens beta-naphthol + acetate SDS-PAGE and in gel-zymography ?
3.1.1.6 phenyl acetate + H2O sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8, 3 min, 1 mM CaCl2 or 5 mM EDTA Homo sapiens phenol + acetate spectrophotometry, absorbance at 270 nm ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.6 C20orf3
-
 Homo sapiens