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Literature summary extracted from

  • Calamini, B.; Santarsiero, B.D.; Boutin, J.A.; Mesecar, A.D.
    Kinetic, thermodynamic and X-ray structural insights into the interaction of melatonin and analogues with quinone reductase 2 (2008), Biochem. J., 413, 81-91.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.5.1
-
Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.10.5.1 the crystal structure of melatonin and 2-iodomelatonin in complex with quinone reductase 2 provide a detailed description of the enzyme active site that can now be utilized in the design of new and potent inhibitors of the enzyme, hanging-drop vapour-diffusion method Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.5.1 2-iodomelatonin
-
Homo sapiens
1.10.5.1 5-hydroxytryptamine
-
Homo sapiens
1.10.5.1 Melatonin competitive inhibitor against N-methyldihydronicotinamide, uncompetitive against menadione. Melatonin and its analogues bind to and inhibit QR2 within the active site and not at an allosteric site Homo sapiens
1.10.5.1 N-acetyl-5-hydroxytryptamine
-
Homo sapiens
1.10.5.1 resveratrol
-
Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.5.1 cytosol
-
Homo sapiens 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.10.5.1 Homo sapiens P16083 recombinant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.5.1
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.5.1 N-methyldihydronicotinamide + menadione
-
Homo sapiens N-methylnicotinamide + menadiol
-
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Synonyms

EC Number Synonyms Comment Organism
1.10.5.1 MT3
-
Homo sapiens
1.10.5.1 QR2
-
Homo sapiens
1.10.5.1 quinone reductase 2
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.5.1 25
-
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.5.1 8
-
-
Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.10.5.1 0.0072
-
Melatonin pH 8, 25°C, competitive inhibitor against N-methyldihydronicotinamide Homo sapiens
1.10.5.1 0.092
-
Melatonin pH 8, 25°C, uncompetitive against menadione Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.10.5.1 0.00096
-
pH 8, 25°C Homo sapiens resveratrol
1.10.5.1 0.0011
-
pH 8, 25°C Homo sapiens 2-iodomelatonin
1.10.5.1 0.0099
-
pH 8, 25°C Homo sapiens N-acetyl-5-hydroxytryptamine
1.10.5.1 0.0113
-
pH 8, 25°C Homo sapiens Melatonin
1.10.5.1 0.0539
-
pH 8, 25°C Homo sapiens 5-hydroxytryptamine