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Literature summary extracted from

  • Staab, C.A.; Alander, J.; Brandt, M.; Lengqvist, J.; Morgenstern, R.; Grafstroem, R.C.; Hoeoeg, J.O.
    Reduction of S-nitrosoglutathione by alcohol dehydrogenase 3 is facilitated by substrate alcohols via direct cofactor recycling and leads to GSH-controlled formation of glutathione transferase inhibitors (2008), Biochem. J., 413, 493-504.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.284 formaldehyde in the presence of NAD+ and initial absence of NADH, ADH3-mediated S-nitrosoglutathione reduction is triggered by the presence of formaldehyde Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.284 expressed in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.00012
-
 S-hydroxymethylglutathione in 0.1 M potassium phosphate (pH 7.5), at 25°C Homo sapiens
1.1.1.284 0.0111
-
 S-nitrosoglutathione in 0.1 M potassium phosphate (pH 7.5), at 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284 Blue-Sepharose 6 column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Homo sapiens S-formylglutathione + NADH + H+
-
 r
1.1.1.284 S-hydroxymethylglutathione + NAD+
-
 Homo sapiens S-formylglutathione + NADH + H+
-
 r
1.1.1.284 S-nitrosoglutathione + NADH + H+
-
 Homo sapiens S-amino-L-glutathione + NAD+ + ?
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.284 ADH3
-
 Homo sapiens
1.1.1.284 alcohol dehydrogenase 3 exhibits dual function as S-nitrosoglutathione reductase and glutathione-dependent formaldehyde dehydrogenase Homo sapiens
1.1.1.284 GSNO reductase
-
 Homo sapiens
1.1.1.284 GSNOR
-
 Homo sapiens
1.1.1.284 S-nitrosoglutathione reductase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.284 2.5
-
 S-hydroxymethylglutathione in 0.1 M potassium phosphate (pH 7.5), at 25°C Homo sapiens
1.1.1.284 20
-
 S-nitrosoglutathione in 0.1 M potassium phosphate (pH 7.5), at 25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 Homo sapiens