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Literature summary extracted from

  • Tan, F.; Cheng, Q.; Saha, K.; Heinemann, I.U.; Jahn, M.; Jahn, D.; Smith, A.G.
    Identification and characterization of the Arabidopsis gene encoding the tetrapyrrole biosynthesis enzyme uroporphyrinogen III synthase (2008), Biochem. J., 410, 291-299.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.75 gene HEM4, library screening and complementation studies, sequence comparisons, expression of the enzyme in the knockout Saccharomyces cerevisiae S150-2B strain, overvexpression of the His-tagged enzyme in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.75 chloroplast exclusive localization in chloroplasts, UROS has an N-terminal extension acting as a targeting sequence for import of the precursor protein of 34 kD into chloroplasts, followed by processing to the mature size of 29 kDa Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.75 29000
-
 x * 34000, precursor enzyme, x * 29000, mature enzyme Arabidopsis thaliana
4.2.1.75 34000
-
 x * 34000, precursor enzyme, x * 29000, mature enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.75 hydroxymethylbilane Arabidopsis thaliana a step in tetrapyrrole biosynthesis, e.g. of chlorophyll, haem, sirohaem and bilins, overview uroporphyrinogen III + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.75 Arabidopsis thaliana O48721
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.1.75 proteolytic modification the N-terminal extension of UROS is processed resulting in the mature enzyme of 29 kDa Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.75 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration to homogeneity Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.75 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.75 hydroxymethylbilane a step in tetrapyrrole biosynthesis, e.g. of chlorophyll, haem, sirohaem and bilins, overview Arabidopsis thaliana uroporphyrinogen III + H2O
-
 ?
4.2.1.75 hydroxymethylbilane uroporphyrinogen III synthase catalyses the cyclization of HMB with a concomitant inversion of the fourth ring of the porphyrin macrocycle, giving rise to uroporphyrinogen III Arabidopsis thaliana uroporphyrinogen III + H2O
-
 ?
4.2.1.75 additional information coupled assay method for UROS activity measurement using purified recombinant Pseudomonas aeruginosa porphobilinogen synthase and Bacillus megaterium porphobilinogen deaminase to generate hydroxymethylbilane, the substrate for UROS, enzymatically from 5-aminolaevulinic acid Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.75 ? x * 34000, precursor enzyme, x * 29000, mature enzyme Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
4.2.1.75 Uroporphyrinogen III synthase
-
 Arabidopsis thaliana
4.2.1.75 UROS
-
 Arabidopsis thaliana