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Literature summary extracted from

  • Barson, H.V.; Mollenkopf, H.; Kaufmann, S.H.; Rijpkema, S.
    Anthrax lethal toxin suppresses chemokine production in human neutrophil NB-4 cells (2008), Biochem. Biophys. Res. Commun., 374, 288-293.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.24.83 diagnostics the development, performance characteristics and validation using human serum of a robust and rugged format of the LTx neutralization activity (TNA) assay is reported and its application in evaluating immune serum from humans, Rhesus macaques and rabbits. This format uses standardized and characterized reagents in conjunction with customized interpretive software and a novel mathematical algorithm to calculate and extrapolate multiple reportable values, in addition to ED50, with high specificity, analytical sensitivity, accuracy and precision. This LTx neutralization activity (TNA) assay format is proposed as a unifying platform technology Bacillus anthracis
3.4.24.83 molecular biology microarray analysis is used to investigate the effects of Bacillus anthracis lethal toxin on human neutrophil-like NB-4 cells to identify markers of intoxication. Genes down-regulated after a 2 h lethal toxin exposure include those encoding chemokines and transcription factors. Significant decreases in the mRNA of interleukin-8, CCL20, CCL3 and CCL4 are observed using real-time PCR. The decreases are more pronounced at 4 and 8 h and are lethal toxin-specific. Decreases in chemokine protein levels are evident after 24 h and are sensitive to low concentrations of lethal toxin. Co-incubation with an anti-lethal factor mAb restores levels of interleukin-8 to 100% and 50%, respectively Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.83 Bacillus anthracis
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Source Tissue

EC Number Source Tissue Comment Organism Textmining

Synonyms

EC Number Synonyms Comment Organism
3.4.24.83 anthrax lethal toxin
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Bacillus anthracis
3.4.24.83 LTx
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Bacillus anthracis