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Literature summary extracted from

  • Hehemann, J.H.; Redecke, L.; Murugaiyan, J.; von Bergen, M.; Betzel, C.; Saborowski, R.
    Autoproteolytic stability of a trypsin from the marine crab Cancer pagurus (2008), Biochem. Biophys. Res. Commun., 370, 566-571.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.4 isozyme TryIII displays high resistance against autoproteolysis with a low susceptibility of the N-terminal domain towards autolysis Cancer pagurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 4-(2-aminoethyl)-benzensulfonylfluoride isozyme TryIII activity is completely (more than 95%) inhibited by 4-(2-aminoethyl)-benzensulfonylfluoride Cancer pagurus
3.4.21.4 additional information aspartate (PepA) and cysteine (E64) protease inhibitors as well as the chymotrypsin inhibitor tosyl-L-phenylalanine chloromethylketone have no effect on enzymatic activity of isozyme TryIII Cancer pagurus
3.4.21.4 N-alpha-tosyl-L-lysine chlormethylketone less than 50% inhibition of isozyme TryIII by the trypsin-specific inhibitor N-alpha-tosyl-L-lysine chlormethylketone Cancer pagurus
3.4.21.4 Soybean trypsin inhibitor isozyme TryIII activity is completely (more than 95%) inhibited by soybean trypsin inhibitor Cancer pagurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.4 25700
-
 isozyme TryIII, calculated from amino acid sequence Cancer pagurus
3.4.21.4 26000
-
 isozyme TryIII, SDS-PAGE Cancer pagurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Cancer pagurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4 NAP-10 Sephadex G25 gel filtration and UNO Q6 column chromatography Cancer pagurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 gastric juice
-
 Cancer pagurus
-
3.4.21.4 hepatopancreas
-
 Cancer pagurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
-
 Cancer pagurus N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.4 TryI isozyme Cancer pagurus
3.4.21.4 TryII isozyme Cancer pagurus
3.4.21.4 TryIII isozyme Cancer pagurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.4 Cancer pagurus isozyme TryIII, calculated from amino acid sequence
-
 4.4