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Literature summary extracted from

  • Chen, S.; Lai, M.; Lai, S.; Lee, Y.
    Characterization of osmolyte betaine synthesizing sarcosine dimethylglycine N-methyltransferase from Methanohalophilus portucalensis (2009), Arch. Microbiol., 191, 735-743.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.157 betaine above 1.0 M, no effect on sarcosine N-methyltransferase activity, 35% inhibition of N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
2.1.1.157 additional information potassium or sodium ions at 200-1000 mM affect neither sarcosine N-methyltransferase activity nor dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
2.1.1.157 S-adenosyl-L-homocysteine 0.2 mM, 63% loss of sarcosine N-methyltransferase activity, 81% loss of dimethylglycine N-methyltransferase activity. At 1 mM, complete loss of sarcosine N-methyltransferase activity, 88% loss of dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.157 0.21
-
 S-adenosyl-L-methionine cosubstrate sarcosine, pH 7.2, 37°C Methanohalophilus portucalensis
2.1.1.157 0.59
-
 S-adenosyl-L-methionine cosubstrate N,N-dimethylglycine, pH 7.2, 37°C Methanohalophilus portucalensis
2.1.1.157 2.29
-
 sarcosine pH 7.2, 37°C Methanohalophilus portucalensis
2.1.1.157 3.76
-
 N,N-dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.157 33000
-
 gel fitlration Methanohalophilus portucalensis

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.157 Methanohalophilus portucalensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.157
-
 Methanohalophilus portucalensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.157 0.71
-
 pH 7.2, 37°C, sarcosine N-methyltransferase activity Methanohalophilus portucalensis
2.1.1.157 3
-
 pH 7.2, 37°C, N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis

Storage Stability

EC Number Storage Stability Organism
2.1.1.157 -85°C, 5 months, no significant loss of both activities Methanohalophilus portucalensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.157 S-adenosyl-L-methionine + N,N-dimethylglycine
-
 Methanohalophilus portucalensis S-adenosyl-L-homocysteine + betaine + H+
-
 ?
2.1.1.157 S-adenosyl-L-methionine + sarcosine about 30% of the activity with N,N-dimethylglycine Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.157 monomer 1 * 33000, SDS-PAGE Methanohalophilus portucalensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.157 0.44
-
 sarcosine pH 7.2, 37°C Methanohalophilus portucalensis
2.1.1.157 2.68
-
 N,N-dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.157 Methanohalophilus portucalensis isoelectric focusing
-
 5