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Literature summary extracted from

  • Tobimatsu, T.; Nishiki, T.; Morimoto, M.; Miyata, R.; Toraya, T.
    Low-solubility glycerol dehydratase, a chimeric enzyme of coenzyme B(12)-dependent glycerol and diol dehydratases (2008), Arch. Microbiol., 191, 199-206.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.28 beta-subunit DNA and amino acid sequence determination and analysis, sequence comparison, and expression of wild-type and chimeric mutants, in Escherichia coli strain BL21(DE3), distribution of the chimeric mutants in recombinant bacterial cells, overview Klebsiella pneumoniae
4.2.1.28 beta-subunit DNA and amino acid sequence determination and analysis, sequence comparison, and expression of wild-type and chimeric mutants, in Escherichia coli strain BL21(DE3), distribution of the chimeric mutants in recombinant bacterial cells, overview Salmonella enterica
4.2.1.28 beta-subunit DNA and amino acid sequence determination and analysis, sequence comparison, and expression of wild-type and chimeric mutants, in Escherichia coli strain BL21(DE3), distribution of the chimeric mutants in recombinant bacterial cells, overview Klebsiella oxytoca

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.28 additional information construction of chimeric enzymes with subunit compositions alphaGbetaD2GgammaG and alphaGbetaGgammaDG, overview Klebsiella pneumoniae
4.2.1.28 additional information construction of chimeric enzymes with subunit compositions alphaGbetaD2GgammaG and alphaGbetaGgammaDG, overview Salmonella enterica
4.2.1.28 additional information construction of chimeric enzymes with subunit compositions alphaGbetaD2GgammaG and alphaGbetaGgammaDG, overview Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.28 additional information Klebsiella pneumoniae the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes ?
-
 ?
4.2.1.28 additional information Salmonella enterica the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes ?
-
 ?
4.2.1.28 additional information Klebsiella oxytoca the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Klebsiella oxytoca
-
-
-
4.2.1.28 Klebsiella pneumoniae
-
-
-
4.2.1.28 Salmonella enterica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-propanediol
-
 Klebsiella pneumoniae propanal + H2O
-
 ?
4.2.1.28 1,2-propanediol
-
 Salmonella enterica propanal + H2O
-
 ?
4.2.1.28 1,2-propanediol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
4.2.1.28 additional information the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes Klebsiella pneumoniae ?
-
 ?
4.2.1.28 additional information the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes Salmonella enterica ?
-
 ?
4.2.1.28 additional information the enzyme catalyze the conversion of 1,2-diols, such as 1,2-propanediol, ethanediol and glycerol, to the corresponding aldehydes Klebsiella oxytoca ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.28 diol dehydratase
-
 Klebsiella pneumoniae
4.2.1.28 diol dehydratase
-
 Salmonella enterica
4.2.1.28 diol dehydratase
-
 Klebsiella oxytoca
4.2.1.28 DL-1,2-propanediol hydro-lyase
-
 Klebsiella pneumoniae
4.2.1.28 DL-1,2-propanediol hydro-lyase
-
 Salmonella enterica
4.2.1.28 DL-1,2-propanediol hydro-lyase
-
 Klebsiella oxytoca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.28 37
-
 assay at Klebsiella pneumoniae
4.2.1.28 37
-
 assay at Salmonella enterica
4.2.1.28 37
-
 assay at Klebsiella oxytoca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.28 8
-
 assay at Klebsiella pneumoniae
4.2.1.28 8
-
 assay at Salmonella enterica
4.2.1.28 8
-
 assay at Klebsiella oxytoca