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Literature summary extracted from

  • Thompson, H.; Tersteegen, A.; Thauer, R.; Hedderich, R.
    Two malate dehydrogenases in Methanobacterium thermoautotrophicum (1998), Arch. Microbiol., 170, 38-42.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.299 NADH inhibitory above 0.2 mM Methanothermobacter thermautotrophicus
1.1.1.299 NADPH inhibitory above 0.2 mM Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.299 0.09
-
 NADPH pH 7.6 Methanothermobacter thermautotrophicus
1.1.1.299 0.09
-
 NADH pH 7.6 Methanothermobacter thermautotrophicus
1.1.1.299 0.09
-
 oxaloacetate pH 7.6 Methanothermobacter thermautotrophicus
1.1.1.299 0.4
-
 malate pH 7.6 Methanothermobacter thermautotrophicus
1.1.1.299 0.4
-
 malate at pH 7.6 and 60°C Methanothermobacter thermautotrophicus
1.1.1.299 0.5
-
 NAD+ pH 7.6 Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.299 36000
-
 x * 36000, SDS-PAGE, x * 36700, calculated Methanothermobacter thermautotrophicus
1.1.1.299 36700
-
 x * 36000, SDS-PAGE, x * 36700, calculated Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.299 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.299
-
 Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.299 1
-
 pH 7.6, oxidation of malate, cosubstrate NAD+ Methanothermobacter thermautotrophicus
1.1.1.299 1
-
 pH 7.6, oxidation of malate, cosubstrate NADP+ Methanothermobacter thermautotrophicus
1.1.1.299 52
-
 pH 7.6, reduction of oxaloacetate, cosubstrate NADP+ Methanothermobacter thermautotrophicus
1.1.1.299 60
-
 pH 7.6, reduction of oxaloacetate, cosubstrate NAD+ Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.299 (S)-malate + NAD+
-
 Methanothermobacter thermautotrophicus oxaloacetate + NADH + H+
-
 ?
1.1.1.299 (S)-malate + NADP+
-
 Methanothermobacter thermautotrophicus oxaloacetate + NADPH + H+
-
 ?
1.1.1.299 additional information no substrate: pyruvate Methanothermobacter thermautotrophicus ?
-
 ?
1.1.1.299 oxaloacetate + NADH
-
 Methanothermobacter thermautotrophicus L-malate + NAD+
-
 ?
1.1.1.299 oxaloacetate + NADPH
-
 Methanothermobacter thermautotrophicus L-malate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.299 ? x * 36000, SDS-PAGE, x * 36700, calculated Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.299 MDH II uses both NADP+ and NAD+ Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.299 NAD+
-
 Methanothermobacter thermautotrophicus
1.1.1.299 NADH
-
 Methanothermobacter thermautotrophicus
1.1.1.299 NADP+
-
 Methanothermobacter thermautotrophicus
1.1.1.299 NADPH
-
 Methanothermobacter thermautotrophicus