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Literature summary extracted from

  • Carbone, V.; Chung, R.; Endo, S.; Hara, A.; El-Kabbani, O.
    Structure of aldehyde reductase in ternary complex with coenzyme and the potent 20alpha-hydroxysteroid dehydrogenase inhibitor 3,5-dichlorosalicylic acid: implications for inhibitor binding and selectivity (2008), Arch. Biochem. Biophys., 479, 82-87.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.2 at 22°C by vapor-diffusion using the hanging drop method. ALR1 in ternary complex with the coenzyme NADPH and 3,5-dichlorosalicylic acid, at a resolution of 2.41 A Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.2 20alpha-hydroxysteroid dehydrogenase competitive inhibition, the inhibitor forms a 10fold stronger binding interaction with the catalytic residue (Tyr55), non-conserved hydrogen bonding interaction with His222, and additional van der Waals contacts with the non-conserved C-terminal residues Leu306, Leu308 and Phe311 that contribute to the inhibitor's selectivity advantage for 20alpha-hydroxysteroid dehydrogenase over 3,5-dichlorosalicylic acid Sus scrofa
1.1.1.2 3,5-dichlorosalicylic acid mixed type of competitive and non-competitive patterns with respect to the substrate. The inhibitor forms a network of hydrogen bonds with the active site residues Trp22, Tyr50, His113, Trp114 and Arg312. Is a less potent inhibitor of ALR1 (256fold) when compared to 20alpha-hydroxysteroid dehydrogenase Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Sus scrofa P50578
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2 to homogeneity Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.2 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 DL-glyceraldehyde + NADPH + H+
-
 Sus scrofa glycerol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 aldehyde reductase
-
 Sus scrofa
1.1.1.2 ALR1
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADPH
-
 Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.2 0.000044
-
-
 Sus scrofa 20alpha-hydroxysteroid dehydrogenase
1.1.1.2 0.011
-
-
 Sus scrofa 3,5-dichlorosalicylic acid